Handbook of Meat Processing

(Greg DeLong) #1
Species Origin of spontaneously
fermented sausages

Identifi cation approach

Pd. acidilactici Italy
Argentina
Spain

PCR - DGGE 3, 15
Phenotypic 10
SDS - PAGE - sequencing of 16S - rRNA gene 20
Pd. pentosaceus Italy Phenotypic 10
Ws. hellenica Italy Species specifi c PCR 1
PCR - DGGE 1
Ws. paramesenteroides Italy
Greece

Species specifi c PCR 1
PCR - DGGE 1
Sequencing of 16S - rRNA gene 5
Ws. paramesenteroides/
hellenica

Greece
Hungary
Italy

Sequencing of 16S - rRNA gene 5
PCR - DGGE 7, 16

Ws. viridescens Greece
Hungary

Sequencing of 16S - rRNA gene 5
PCR - DGGE 7
St. aureus Spain SDS - PAGE - sequencing of 16S - rRNA gene 18
St. auricularis serbian Phenotypic 10
St. capitis Serbia
Croatia

Phenotypic 10

St. caprae Greece
Bosnia - Herzegovina

Phenotypic 10

St. carnosus Slovakia
Spain
Croatia
Italy

Species specifi c PCR 2,11
16S – 23S rDNA intergenic region amplifi cation —
species specifi c PCR 9
Phenotypic 10
St. cohnii Italy PCR - DGGE 11
St. cohnii cohnii Greece Phenotypic 24
St. cohnii urealyticum Greece Phenotypic 10
St. epidermidis Spain
Bosnia - Herzegovina
Italy

16S – 23S rRNA intergenic region amplifi cation - species
specifi c PCR 9
Phenotypic 10
Species specifi c PCR 11
SDS - PAGE - sequencing of 16S - rRNA gene 18
St. equorum Italy
France
Argentina
Spain

PCR - DGGE 6,11,14, 15, 23
PFGE - Sequencing of sodA int gene 8
SDS - PAGE - sequencing of 16S - rRNA gene 18
RAPD - PCR - sequencing of 16S - rRNA gene 21
St. gallinarum Greece Phenotypic 24
St. haemolyticus Italy PCR - DGGE 6
St. hominis Greece
Italy

Phenotypic 10

St. lentus Hungary
Italy

Phenotypic 10

St. pasteuri Italy PCR - DGGE 6, 11
St. saprophyticus Italy
Greece
Boznia - Herzegovina
Croatia
Argentina
Spain

PCR - DGGE 6, 14, 15
Phenotypic 10, 24
RAPD - PCR - sequencing of 16S - rRNA gene 14
SDS - PAGE - sequencing of 16S - rRNA gene 18

Table 9.1. Microbial diversity in spontaneously fermented sausages throughout the world (cont.)


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