Quick Review Cards for Medical Laboratory Science

Speciation of Haemophilus Clinical Microbiology Review 195

REQUIRES X FACTOR REQUIRES V HEMOLYSIS ON RABBIT

SPECIES (HEMIN) FACTOR (NAD) OR HORSE BLOOD AGAR PORPHYRIN/ALA

H. influenzae &H. aegyptius ++ 0 0

H. parainfluenzae 0+ 0 +

H. haemolyticus ++ + 0

H. parahaemolyticus 0+ + +

H. ducreyi +0 0 0

Note that porphyrin/ALA reactions & X factor requirement are opposite, i.e., species that are pos for porphyrin/ALA don’t require X factor. Species with “para” in name only
require V factor & are porphyrin/ALA pos. Species with “haemolyticus” in name are hemolytic on rabbit & horse blood agar. Multitest biochemical systems available for ID.