Blood Components—Platelets Immunohematology Review 430
STORAGE
COMPONENT PREPARATION TEMPERATURE SHELF LIFE INDICATIONS OTHER
Platelets
Apheresis platelets
Leukocyte-reduced
platelets
Prestorage pooled
platelets
40–70 mL plasma.≥5.5
¥ 1010 plt. pH ≥6.2.
1 unit should ↑plt by
5,000–10,000/μL in 75-kg
recipient. Shouldn’t be
used if visible aggregates
present. May contain
residual RBCs. Usually
pooled (4–6 units).
≥3.0 ¥ 1011 plt. Equiva-
lent to 4–6 units. Exposes
recipient to fewer donors.
Extended outdate over-
comes need for tf service
to pool just before
administration.
Centrifugation of
WB at RT within
8 hr of collection.
1st soft spin
yields platelet-
rich plasma.
2nd hard spin
separates plt
from plasma.
Apheresis.
WBCs removed
by filtration or
during apheresis
processing.
4–6 ABO-
identical plt
pooled using
closed system.
20 °–24°C
20 °–24°C
20 °–24°C
20 °–24°C
5 days from collection,
with agitation. After
pooling, 4 hr
5 days with agitation
Open system—4 hr.
Apheresis—5 days
5 days from collection
Severe thrombocy-
topenia or abnormal
platelet function
Same
Recurrent febrile rxn
& to ↓risk of CMV
transmission or HLA
alloimmunization
Same
