Quick Review Cards for Medical Laboratory Science

Semen Analysis

Urinalysis and Body

Fluids Review 521

FERTILITY TESTING POST-VASECTOMY

Specimen collection

Liquefaction

Volume

Motility

Cell count

Morphology

pH

Condom can be used. Time & temp not critical.

Test monthly beginning 2 months after vasec-

tomy. Continue until 2 consecutive specimens

are without sperm.

Don’t analyze until specimen is liquified.

NA.

NA.

Examine undiluted & following centrifugation.

Even 1 sperm is significant.

NA.

Collect in sterile container, without condom, after

3-day abstinence. Keep at RT. Deliver to lab within

1 hr of collection.

Don’t analyze until specimen is liquified. (Normally

within 30 min of collection.)

Normal: 2–5 mL

Observe within 3 hr of collection. 50%–60% of sperm

should show at least fair motility.

Dilute & count in Neubauer hemacytometer.

Normal: >20 million per mL.

Stain & examine at least 200 cells. Normal: oval head

(3 × 5 μm) with long tapering tail. Abnormalities:

double heads, giant heads, amorphous heads, pinheads,

tapering heads, constricted heads, double tails, coiled

tails, large numbers of spermatids (immature forms).

Normal = <30% abnormal forms.

7.3–8.3