- Which of the following infection(s) can be diagnosed
by the use of polymerase chain reaction (PCr)?
(a) HIV-1 and HIV-2 viruses
(b) Hepatitis-b virus
(c) Mycobacterium tuberculosis
(d) all of the above
- Vent DNa polymerase enzyme used in PCr is isolated
from
(a) Thermococcus litoralis
(b) Thermus aquaticus
(c) E. coli
(d) Salmonella typhimurium.
- Match the following columns.
Column-I Column-II
a. Isolation of genetic 1. Separation and
material purification of the
product
b. fragmentation of DNa 2. recombinant protein
C. Isolation of desired 3. Transformation
DNa fragment
D. amplification of gene of 4. DNa ligase
interest
e. Ligation of the DNa 5. Polymerase chain
fragment into a vector reaction
f. Insertion of recombinant 6. agarose gel
DNa into the host electrophoresis
G. extraction of desired 7. restriction enzymes
gene product
H. Downstream processing 8. ethanol
A B C D E F G H
(a) 5 4 3 2 1 6 8 7
(b) 8 7 6 5 4 3 2 1
(c) 7 8 5 6 4 3 1 2
(d) 1 2 3 5 4 6 8 7
- electroporation involves
(a) promotion of seed germination by induced
imbibition of water with electric current
(b) making transient pores in cell membrane to
facilitate entry of gene constructs
(c) purification of saline water with the help of an
artificial membrane
(d) passage of sucrose through sieve pores by
electroosmosis.
- Main objective of production/use of herbicide resistant
GM crops is to
(a) encourage eco-friendly herbicides
(b) reduce herbicide accumulation in food articles for
health safety
(c) eliminate weeds from the field without the use of
manual labour
(d) eliminate weeds from the field without the use of
herbicides.
- Plasmid used to construct the first recombinant DNa
was isolated from which bacterium species?
(a) Escherichia coli
(b) Salmonella typhimurium
(c) Agrobacterium tumefaciens
(d) Thermus aquaticus
- Genetic engineering is possible, because
(a) we can cut DNa at specific sites by endonucleases
like DNaase I
(b) restriction endonucleases purified from bacteria
can be used in vitro
(c) the phenomenon of transduction in bacteria is
well understood
(d) we can see DNa by electron microscope.
- Variable number of tandem repeats (VNTrs) in the DNa
molecule are highly useful in
(a) recombinant DNa technology
(b) DNa finger printing
(c) monoclonal antibody production
(d) stem cell culture.
- The restriction enzymes are used in genetic engineering,
because they
(a) can degrade harmful proteins
(b) can cut DNa at specific base sequence
(c) can join different DNa fragments
(d) are nucleases that cut DNa at variable sites.
- Who discovered recombinant DNa (rDNa) technology?
(a) Har Gobind Khurana
(b) James D Watson
(c) Stanley Cohen and Herbert boyer
(d) Walter Sutton and avery
- a student performed the following steps in a Southern
blot experiment to determine the number of copies of
a particular gene that has been inserted in a genetically
modified organism.
(i) Transfer of DNa to nitrocellulose membrane.
(ii) restriction digestion of genomic DNa.
(iii) Cleaved DNa separated using gel electrophoresis.
(iv) Create radioactive probe.
(v) Incubate probe and membrane.
Which is the correct sequence to the above steps?
(a) (ii) → (iii) → (i) → (iv) → (v)
(b) (ii) → (iii) → (i) → (v) → (iv)
(c) (iv) → (v) → (i) → (ii) → (iii)
(d) (v) → (iv) → (iii) → (ii) → (i)
