Cell - 8 September 2016
intracellular zinc chaperones, are highly enriched in the most dysfunctional CD8+TILs and demonstrate that targeted deletion of ...
T cells and transferred these cells or control OT-1 CD8+T cells into wild-type (WT) mice bearing MC38-Ova tumors. Recipients of ...
Metallothionein Deficiency Uncouples Co-inhibitory Receptor Expression from a T Cell Dysfunction Phenotype We next analyzed the ...
effector function and retarded tumor growth inMT/mice, Tim- 3 and PD-1 expression was either unchanged or even increased onMT ...
signatures and memory CD8+T cell signatures (STAR Methods) (Eden et al., 2007; Wagner, 2015). Accordingly, we termed it a naive/ ...
single-cell RNA-seq (Tirosh et al., 2016). In human melanoma TILs, we found evidence for a similar phenomenon with genes in the ...
expression of one module’s genes by a cell predicts lower expression of the other module’s genes in the same cell. Similarly, th ...
Gata-3 Regulates Dysfunction in CD8+TILs To validate that members of the dysfunctional signature perform important functions and ...
B D E G C F 6.99 0 10,000 20,000 30,000 40,000 * 0 20 40 60 80 *** 0 2 4 6 8 * IL-2 + of CD8 IL-10 T-cells (%) + of CD8 T-cells ...
one can envision how disruption of intracellular zinc availability can impact the structure and activity of multiple proteins th ...
of CD8+ T cell exhaustion by multiple inhibitory receptors during chronic viral infection. Nat. Immunol. 10 , 29–37. Bonaventura ...
STAR+METHODS KEY RESOURCES TABLE REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat anti-PD-1 (clone: RMP1-30) Biolegend Cat#1 ...
CONTACT FOR REAGENT AND RESOURCE SHARING Requests of reagents should be directed to Ana C. Anderson
[email protected]
. E ...
Generation of Lentiviral Constructs and CRISPR-Cas9 Targeting The initial guide sequences were selected based on the exon struct ...
To profile the RNA of in vitro activated CD8+T cells, we isolated naive CD8+cells from non-tumor-bearing C57BL/6 mice and acti- ...
We filtered out low quality cells and cell doublets, maintaining for subsequent analysis the 1,061 cells (516 WT and 545MT/) t ...
Supplemental Figures Figure S1. Expression Trends of CD8+TILs Clusters, Related toFigure 1 Each of the ten clusters obtained (se ...
A B C 0 5000 10,000 15,000 20,000 MT1: CT26 0 2000 4000 6000 8000 10,000 MT1: B16 0 2000 4000 6000 MT2: CT26 0 500 105 105 104 1 ...
Figure S3. Overlay of Naive and In Vitro Activated CD8+T Cell Transcriptomes on PC1 and PC2, Related toFigure 3 Naive CD8+CD62Lh ...
***p-value < 0.001 −5 0 5 10 TNF IFNG GZMM GZMC GZMF GZMA PRF1 GZMK GZMB Cluster 5 Cluster 7 Centered normalized expression * ...
«
10
11
12
13
14
15
16
17
18
19
»
Free download pdf