***p-value < 0.001−5 0 5 10TNFIFNGGZMMGZMCGZMFGZMAPRF1GZMKGZMBCluster 5 Cluster 7Centered normalized expression************p-value < 0.05Figure S4. Cytokine and Effector Molecule Expression in Single-Cell Clusters 7 and 5, Related toFigure 5
Centered and normalized RNA levels are shown for different cytokines or effector molecules (rows) for each of the cells (columns) in clusters 5 and 7 from the
single-cell analysis ofFigure 5. To correct for differences in library complexity between cells and allow a comparison at the single-gene level, expression levels for
all genes and cells analyzed (Figure 5) were normalized by partitioning cells into 10 bins by their library complexity and conducting a median-normalization
procedure for each gene, as previously described (Gaublomme et al., 2015). IL2, GZMD and GZME are not included in this analysis because they did not pass the
required expression thresholds to be included in the overall single-cell analysis (STAR Methods). *p < 0.05, ***p < 0.001. Overall p value for cytokine/effector
molecule signature was p < 10^8 , (Wilcoxon rank sum test).