Cell - 8 September 2016
Figure S3. Construction and Validation of the Cas9 Strain Expressing H2B-YFP, Related toFigure 5 (A) Diagram of the constructs u ...
Figure S4. Alignment of CLAMP Orthologs, Related toFigure 6 CLAMP orthologs representing the entire breadth of the apicomplexan ...
Figure S5. Extended Analysis of CLAMP inT. gondiiandP. falciparum, Related toFigure 6 (A) Localization of CLAMP After Invasion. ...
Article Structure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex Graphical Ab ...
Article Structure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex Tiago R.D. C ...
elucidating this interaction is the determination of its structural basis. However, while rapid progress in the structural biolo ...
above by a rotation angle and rise (Figures 2C and 2D). For pED208, the angle between adjacent subunits is 28.2and the rise is ...
Structure of the TraA Pilin The pED208 TraA pilin is a 64-residue protein, 63 of which (resi- dues2–64) were clearly defined in ...
The composition of the lumen is unique in being lined not only with residues from thea2-a3 loop, but also with the lipid head gr ...
location of these mutants is shown inFigure S5): A28 makes hydrophobic interactions with the acyl chain of PG and was mutated to ...
proteins but often as a result of unspecific binding. Only lipid metabolizing enzymes form stoichiometric complexes with their s ...
indeed be used to derive effective bacteriophage therapies to combat bacterial infectious diseases. STAR+METHODS Detailed method ...
ACKNOWLEDGMENTS This work was supported by Wellcome Trust grants 098302 to G.W. and 093228 to T.K.S. and NIH grant GM035269 to E ...
STAR+METHODS KEY RESOURCES TABLE CONTACT FOR REAGENT AND RESOURCE SHARING Contact should be directed to Gabriel Waksman atg.waks ...
METHOD DETAILS Pilus Production and Purification The F pilus encoded by the pOX38 plasmid (a gift from Prof. Fernando de la Cruz ...
required for modeling the phospholipid were generated using COOT’s ‘‘Ligand builder’’ tool, and the dictionary file required for ...
QUANTIFICATION AND STATISTICAL ANALYSIS Quantification and statistical analyses employed in this publication pertain to the anal ...
Supplemental Figures Figure S1. Resolution and Model Statistics for the pED208 Pilus Structure, Related toFigure 1 (A and B) (A) ...
Figure S2. Structure of the F Pilus, Related toFigure 1 (A) Electron micrograph of the F pilus. The scale bar represents 40 nm. ...
Figure S3. Structural Comparison of F and pED208 Pilus Subunit and Lipid Structures, Related toFigures 2and 3 (A) Superposition ...
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