Cell - 8 September 2016

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Control (EV) pod-2 fasn-1 haf-1

GFP Fluorescence (RFU)

Control

Heat Shock

A

D

hsp-6p::GFP hsp-60p::GFP

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hsp-6p::GFP hsp-60p::GFP

GFP Fluorescence (RFU)

Control

PHX

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**

B

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DMSO PHX

Triglyceride (mM)

C **

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Control (EV) EV:hsp-6 hsp-6:pod-2 hsp-6:fasn-1

Triglyceride (mM)

*

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EV+DMSO hsp-6 +DMSO EV+PHX hsp-6 +PHX

OCR (pmol\min\10worms)

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E

DMSO PHX DMSO PHX

hsp-16.2p::GFP

(^0) 0 20406080100
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mthsp70 siRNA
(^0020406080100)
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Control siRNA
Time (minutes)
OCR (pmoles/min)
BSA
PALM
Oligo FCCP Rtn/AA
Oligo FCCP Rtn/AA
BSA
PA L M
siRNA: Ctrl mthsp70
mtHSP70
Tubulin
F
Figure S4. Reducing Fat Synthesis Blocks the Cytosolic Response, and Inhibiting CPT Activity Induces the Cytosolic Stress Response,
Related toFigure 4
(A) Triglyceride content was measured after indicated RNAi treatment (paired t test, mean±SD of three biological repeats, *p%0.05).
(B) Knocking down fat synthesis genespod-2andfasn-1had a specific inhibitory effect on mitochondrial to cytosolic signaling while they had no effect on heat
shock response.hsp-16.2p::GFP reporter induction was measured by COPAS biosorter after RNAi and heat shock (mean±SD of three biological repeats).
(C) Triglyceride content was measured after PHX treatment (paired t test, mean±SD of three biological repeats, **p%0.01).
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