Cell - 8 September 2016

(D) PHX induces mitochondrialhsp-6andhsp-60expression.hsp-6p::GFP and reporterhsp-60p::GFP induction was measured by COPAS biosorter after PHX
treatment (mean±SD of three biological repeats, *p%0.05, *p%0.01).
(E) Oxygen consumption rate was measured afterhsp-6RNAi and PHX treatment from whole worms using Seahorse (Paired t test, mean±SD of three biological
repeats, p%0.05).
(F) Oxygen consumption rate was measured with or without fatty acid oxidation substrate after mtHsp70 siRNA treatment in 293T cells. mtHSP70 protein level is
shown after siRNA treatment (inset). Left: respiration in control siRNA treated cells either in the presence of BSA (in blue) or after the addition of the fatty acid
oxidation substrate BSA-palmitate (PALM) (in red), Right: respiration in mtHSP70 siRNA treated cells either in the presence of BSA (in blue) or after the addition of
the fatty acid oxidation substrate BSA-palmitate (PALM) (in red). Oligo; oligomycin, FCCP; carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone, Rtn/AA;
rotenone/antimycin-A.