Cell - 8 September 2016

Figure S5. Cardiolipin Synthesis Is Required for MCSR Induction, and Inhibiting Ceramide Synthesis Resulted in MCSR Induction, Related to
Figure 5
(A) Nonyl acridine orange staining after feeding different types of cardiolipin to the worms.
(B) Induction ofhsp-6p::GFP after feeding the different types of cardiolipin to the reporter worms. Only mild induction in the head and hind-gut was observed.
(C) Nile Red staining after double RNAi.
(D) Ceramide-fed reporter worms in the presence or absence of UPRmt(cco-1RNAi), UPRER(Tunicamycin), and HSR (heat shock) induction.
(E) Upper panel;hsp-16.2p::GFP reporter worms after RNAi of ceramide synthesis genes. Lower panel;hsp-16.2p::GFP reporter worms after double RNAi of
ceramide catabolizing genes withhsp-6RNAi (one to one ratio).
(F) Cardiolipin-fedhsp-16.2::GFP reporter worms. Indicated RNAis were pre-treated at late L3 then transferred to the cardiolipin-containing RNAi plates at L4. The
images were taken 48 hr after feeding the cardiolipin.