Cell - 8 September 2016

A

Control(EV) EV: hsp-6 hsp-6: crls-1RNAi

Nonyl Acridine Orange Staining B Nile Red Staining

Control Heart CL C14 C18

hsp-6p::GFP

Control Heart CL C14 C18

C

D

hsp-6p::GFP

Control Brain CM C16 C20 C22 C24

hsp-4p::GFP

hsp-4p::GFP + 20 ng/μl Tunicamycin

hsp-6p::GFP on cco-1 RNAi

Feeding Ceramides

hsp-16.2p::GFP

hsp-16.2p::GFP + Heat Shock

hsp-16.2p::GFP

EV sptl-1 sptl-2 sptl-3 hyl-1 hyl-2 lagr-1 asm-2 asm-3 ZK856.18RNAi

EV

Double RNAi with hsp-6 RNAi

T10B11.2K11D2.2F27E5.1 W02F12.2

E

F hsp-16.2p::GFP (Cardiolipin feeding on RNAi pre-treated animals)

ControlHeart CL C14 C18 ControlHeart CL C14 C18Control Heart CL C14 C18Control Heart CL C14 C18

Empty Vector dve-1 RNAi hsf-1 RNAi atfs-1 RNAi

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