(C) qPCR of lipid biosynthesis genes from human primary fibroblasts (GFP expressing cells) was performed after mtHSP70 knockdown (mean±SD of three
biological repeats, p%0.05,dp = 0.07).
(D) PolyQ expressing human primary fibroblasts were transfected with siRNAs targetingacc1(#1 and #2) andfas(#1 and #2) in addition tohspa9(mtHSP70)
siRNA (#2). mRNA level from each condition is shown in the right panel (mean±SD of three biological repeats, p%0.05, **p%0.01). PolyQ aggregate formation
is shown in the filter trap in the left panel. For the simplicity, the lane numbers (labeled on the filter trap blot in the left panel) are shown in the total protein control
Western blots and qPCR data.
amelia
(Amelia)
#1