Cell - 8 September 2016

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Figure S5.spg-7Deletion in CRISPR-Cas9 Model Related toFigure 6
(A) A schematic graph of the Cas9-sgRNA plasmid.
(B) A representative DNA gel of the T7EI assay forspg-7PCR products amplified from genomic DNA ofhsp-6p::GFP worms (left) orunc-119p::Cas9+u6p::spg-
7 -sg;hsp-6p::GFP worms with non-autonomous UPRmt(right).
(C) The representative sequence alignments of thespg-7andcco-1genes in wild-type and mutant animals. The PAM sequence is labeled in red. The numbers in
parentheses represent the number of bases not shown. Dash indicates deletion. The number of deleted () or inserted (+) bases is shown on the right of each
indel.