Cell - 8 September 2016

Figure S6. Theoretical Model of the Influence of MEX-5 on Phase Separation of PGL-3 and mRNA, Related toFigures 3, 4 , and 6
(A) Phase diagram and constrained paths due to the binding chemical reactions for various MEX-5 and mRNA concentrations. The symbols denote the points
where cPT= 600 nM for each constrained path.
(B) Fraction of the total fluorescence in drops for each of the constrained paths. To show the correspondence to the respective constrained path in (A),we used
the same symbols; the additional star symbol denotes cMT= 150 nM and cRT= 0 nM. The symbols -/+ indicate absence or presence of PGL-3, MEX-5 and mRNA
at concentrations identical to the experimental study (Figure 4B).
(C and D) Intensity differenceIin–Ioutas a function of droplet volumes. In the absence of mRNA (C), and in the presence of total mouse brain mRNA (50 ng/mL) (D),
the intensity difference changes only weakly (roughly logarithmically) for droplets of different volume V. This indicates that the intensity density inside of drops is
close to the value corresponding of phase separation equilibrium.
(E and F) Results from numerical calculations for the dynamical model with the six components: mRNA, PGL-3, PGL-3:mRNA, MEX-5, MEX-5:mRNA and water.
Snapshots of the full system as a function of time with PGL-3 and PGL-3:mRNA concentrations shown in (E). The remaining concentration fields averaged over
the y- coordinate are depicted in (F).