clusters resulted in higher Mantel statistics
(Fig. 2I) and a stronger dependence between
pairwise Pearson correlations and tuft den-
dritic distance (Fig. 2, E, H, and J to L, and
fig. S4A; linear regression permutation test).
Finally, for the largest-amplitude calcium
events (cluster 4), the activity was spread
throughout the entire tuft tree, and responses
inallROIswerehighlycorrelated(Fig.2,E
and H). This was manifested as lower Mantel
statistics (Fig. 2I) and lower dependence of the
pairwise Pearson correlation values on den-
dritic distance (Fig. 2, E, H, and J to L, and fig.
S4A; linear regression permutation test). Even
in cluster 4 events, it is evident that some
SCIENCEscience.org 15 APRIL 2022¥VOL 376 ISSUE 6590 271
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Soma
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Soma
Soma
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Example 1
A
Example 2
B
Example 3
CD
0.2
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0.6
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Fraction of somatic activation
Whole tree Hemi-tree
100%
Hemi-tree
70-99%
Hemi-tree
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Hemi-tree
10-34%
Soma
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Example 1
E F
0.2
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Fraction of somatic activation
Whole tree Tree
70-99%
Tree
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Type 1 PTNs
Type 2 PTNs
Fig. 4. Tuft and soma activity correlations.Quasi-simultaneous tuft and soma
recordings were performed with an electrically tunable lens (ETL) (~10 Hz) in
type 1 and 2 PTNs. (AtoC) Examples of representativeDF/Ftraces in the tuft
and soma from a type 1 PTN (ROIs are arranged by the tree structure as indicated
by the dendrogram). (D) Mean (±SD) fraction of tuft events that were
simultaneously active with the soma for type 1 PTNs (seven neurons, six animals).
(E) As in (A) for a type 2 PTN. (F) As in (D) for type 2 PTNs (three neurons,
three animals).
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