© Springer International Publishing Switzerland 2016 221
P.V. Pham (ed.), Stem Cell Processing, Stem Cells in Clinical Applications,
DOI 10.1007/978-3-319-40073-0
A
Adipogenesis
cAMP levels , 142
dexamethasone , 142
induction media , 141
induction medium , 153
lipid droplets , 141
quantitative assessment
fl ow cytometry , 143 , 144 , 146
Nile Red , 143 , 145
Oil Red O , 143 , 144 , 154
signalling factors , 140 , 142
Adipose-derived stromal cell (ASC)
absolute cell count , 152 , 154
adipocytes (see Adipogenesis )
adult stem cells , 131
anatomical location , 201
animal models , 149
antigenic features and differentiation
potentials , 201
BAT , 200
beige/brite adipose tissue , 200
energy homeostasis , 201
free fatty acids , 201
functional variability , 202
function studies, loss of , 202
harvesting techniques
Coleman technique , 207–211
dry technique , 206
laser-assisted liposuction , 206
liposuction vs. biopsy/resection ,
204–205
power-assisted liposuction , 206
super-wet technique , 206
ultrasound-assisted liposuction , 206
water-assisted liposuction , 206
wet/tumescent technique , 206
homogeneous cell populations , 202
HSCs , 133
immunophenotypic characterization ,
138–140
in vitro differentiation
adipocytes (see Adipogenesis )
chondrogenesis , 147 , 148 , 155 , 156
myogenesis , 141 , 148
osteogenesis , 145 , 147 , 155
isolation and expansion
AD-SVF , 133 , 151–152 , 211–212
automated closed system , 137 , 138
bone marrow-derived MSCs , 132
centrifugation process , 213
fat processing techniques , 212
fl ow cytometric analysis , 212
gas chromatography , 213
in vitro expansion , 134–136
lipoaspirate , 150 , 151
liposuction , 132 , 215
proliferation , 214
seeding , 133 , 134 , 213–214
subcutaneous vs. visceral , 133
VEGF , 214
ISCT , 132 , 138
patient age and gender , 203–204
pluripotent stem cells , 131
WAT , 200 , 201
yield and growth characteristics , 202
Adipose-derived stromal vascular fraction
(AD-SVF) , 133 , 134
Ageing
adult MSCs , 100