Stem Cell Processing (Stem Cells in Clinical Applications)

222

antioxidants , 74
causes changes , 100
cell surface characterisation , 103
and differentiation , 102 , 103
donor age
age groups , 71
CFU-F data , 72
cultured MSCs , 72
differentiation potential , 72
embryonic stem cells , 99
ex vivo culturing , 74
HLA-2 markers , 100
inner cell mass , 99
osteoarthritis , 101
and proliferation , 101 , 102
regenerative medicine , 100
Alliance for Harmonization of Cellular Therapy
Accreditation (AHCTA) , 24
American Convention on Human Rights
(ACHR) , 7
Ascorbic acid acts , 145
Asia-Pacifi c Economic Cooperation/Life
Sciences Innovation Forum (APEC/
LSIF) , 23
Aspirate , 212

B
Basic fi broblast growth factor (bFGF) , 186
Benchtop fl ow cytometers , 134
Bone marrow-derived mesenchymal stem cell
(BMSCs) , 44 , 101
Brown adipose tissue (BAT) , 200

C
Celution ® system , 137
Centrifugation process , 213
Chimeras , 9
Chondrogenesis , 147 , 148 , 156
Chronic obstructive pulmonary disease
(COPD) , 52
Circumvention tourism , 5
Cluster of differentiation (CD) , 103
Coleman technique , 207
Colony-forming unit-fi broblasts (CFU-F) , 72
Congenital megakaryocytic thrombocytopenia
(CAMT) , 84
Crohn’s disease , 52

D
Diabetes mellitus (DM) , 53 , 90 , 91

E

Embryoid body (EB) formation , 88

Embryonic germ cells (EGC) , 9

Embryonic stem cells (ESCs) , 2 , 9

bioactive scaffold , 194

culture , 186–187

differentiation , 187–189

ethical dilemma , 189

immune isolation , 193

isolation , 184–186

Lentiviruses , 194

pro-survival molecules , 195

somatic cell nuclear transfer , 191–192

sources , 182–184

technical challenges , 189–190

Endoderm differentiation , 91

European Convention on Human Rights

(ECHR) , 7

European Medicines Agency (EMA)

AHCTA , 24

APEC/LSIF , 23

FACT , 24

FDA-EMA-Health Canada ATMP Cluster , 22

future global regulation , 24

IMDRF , 23

RFCTG and RFGTG , 22–23

F

FDA’s Good Laboratory Practice (GLP)

regulations , 190

Flow cytometric analysis , 212

Foetal bovine serum (FBS) , 135

Foetal calf serum (FCS) , 103

Foundation for the Accreditation of Cellular

Therapy (FACT) , 2 4

G

Gas chromatography , 213

Global Harmonization Task Force (GHTF) , 22

Good manufacturing practice (GMP)

guidelines , 190

materials , 111

medicinal and cellular products , 112–114

process , 111

properties , 110

requirements , 112

standardization , 111

traceability , 112

validation , 111

Graft-versus-host disease (GVHD)

treatment , 4 1

Index