Stem Cell Processing (Stem Cells in Clinical Applications)

(Michael S) #1

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Table 2.1

(continued)

Source

Method of isolation

Media

Serum supplement

Cell surface markers

References

Positive

Negative

Sub-amniotic umbilical cord lining membrane

Digestion method

DMEM-HG

FBS

CD29, CD44, CD73, CD90, CD105

CD34, CD45

Kita et al. (

2010

) and

Moretti et al. (

2010

)

DMEM CMRL1660

Synovial fl

uid

Ficoll density gradient method

α-MEM

FBS

CD44, CD90, CD105, CD147, STRO-1

CD31, CD34, CD45, CD106

Morito et al. (

2008

)

Wharton’s jelly

Enzymatic digestion method

DMEM

FBS

CD73, CD90, CD105

CD14, CD34, CD45, CD79, HLA-DR

Hou et al. (

2009

)

Menstrual blood Density gradient

centrifugations

α -MEM

FBS

CD56, CD73, CD90, CD105 and CD146

CD14, CD45, HLA-DR

Rossignoli et al. (

2013

)

Human milk

Centrifugations

DMEM

UCBS

CD44, CD29, SCA-1

CD33, CD34, CD45, CD73

Patki et al. (

2010

)

P.V. P h a m
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