Novel Chemical Tools to Study Ion Channel Biology (Advances in Experimental Medicine and Biology)

114 C. K. McKenzie et al. It is often noted that the fascinating developments leading to the optical control of ion channels sta ...

Flipping the Photoswitch: Ion Channels Under Light Control 115 Cordes T, Heinz B, Regner N, Hoppmann C, Schrader TE, Summerer W, ...

116 C. K. McKenzie et al. Levitz J, Pantoja C, Gaub B, Janovjak H, Reiner A, Hoagland A, Schoppik D, Kane B, Stawski P, Schier A ...

Flipping the Photoswitch: Ion Channels Under Light Control 117 Samanta S, Woolley GA (2011) Bis-azobenzene crosslinkers for phot ...

© Springer Science+Business Media New York 2015 119 C. Ahern, S. Pless (eds.), Novel Chemical Tools to Study Ion Channel Biology ...

120 L. Leisle et al. spectral, photo-activated and bioorthogonal labeling properties. This chapter will focus on three establish ...

Incorporation of Non-Canonical Amino Acids 121 QF$$W51$ 683 JHQHRILQWHUHVW  683  ;ODHYLVRRF\WH H[SUHVVLQJ QF$$FRQWDLQLQJ ...

122 L. Leisle et al. ing, which may be surmountable by some, represent a significant technical barrier to most investigators, an ...

123 Fig. 2 In vivo nonsense suppression. a The non-canonical amino acid ( ncAA) is chemically cou- pled to the dinucleotide phos ...

124 L. Leisle et al. can suppress the opal (TGA) stop codon if the appropriate anti-codon is engineered into the tRNA (Rodriguez ...

125 ncAAs has been optimized (Robertson et al. 1991 ). Protection of the ncAA α-amino group prior to coupling limits reactivity ...

126 L. Leisle et al. yeast PheRS/tRNAPheCUA pair (Furter 1998a). Crucial to success of this approach is the ability to reprogram ...

127 These RS libraries in the presence of the cognate tRNATyrCUA were subjected to a two-step selection process in E. coli (Fig. ...

128 L. Leisle et al. SRVLWLYHVHOHFWLRQ *$/ 7$*7$* '1$ ELQGLQJGRPDLQ WUDQVFULSWLRQDFWLYDWLRQGRPDLQ UHSRUWHUJHQH +,6 85$RU ...

129 cally well characterized and is susceptible to manipulation, qualities that have made it a go-to host organism for directed ...

130 L. Leisle et al. transcriptional activation of URA3, 5-FOA is converted into a cell-toxic product resulting in cell death (F ...

131 2.2.3 Achaeal Pyrrolysyl-tRNA-Synthetase/tRNA Pairs Display Orthogonality in Prokaryotes as well as Eukaryotes The identific ...

132 L. Leisle et al. sion of target genes and ultimately, the expression of an ncAA into a desired protein, can be highly variab ...

133 Lastly, the incorporation of multiple or different ncAAs into the same protein presents a particular challenge. However, the ...

134 L. Leisle et al. A major consideration in using chemical synthesis is the size of the protein. Chemical synthesis is carried ...