Novel Chemical Tools to Study Ion Channel Biology (Advances in Experimental Medicine and Biology)

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Advances in Experimental Medicine and Biology Volume 869 Editorial Board: Irun R. Cohen, The Weizmann Institute of Science, Reho ...

Advances in Experimental Medicine and Biology presents multidisciplinary and dynamic findings in the broad fields of experimenta ...

Christopher Ahern • Stephan Pless Editors Novel Chemical Tools to Study Ion Channel Biology 1 3 ...

ISSN 0065-2598 ISSN 2214-8019 (electronic) Advances in Experimental Medicine and Biology ISBN 978-1-4939-2844-6 ISBN 978-1-4939- ...

v Contents Introduction ...................................................................................................... 1 ...

vii Contributors Christopher A. Ahern Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA, USA ...

viii Contributors Stephan A. Pless Department of Drug Design and Pharmacology, Center for Biopharmaceuticals, University of Cope ...

© Springer Science+Business Media New York 2015 1 C. Ahern, S. Pless (eds.), Novel Chemical Tools to Study Ion Channel Biology, ...

2 S. A. Pless and C. A. Ahern implications of ion channel function on physiological processes are vast. Electri- cal impulses, i ...

Introduction 3 in the light of only 20 naturally occurring amino acids it is not always possible to probe the structural or func ...

4 S. A. Pless and C. A. Ahern recent extension of the basic idea of cysteine modification is described in chapter Bioreactive Te ...

5 C. Grosman () · G. D. Cymes Department of Molecular and Integrative Physiology, Center for Biophysics and Computational Biolo ...

6 G. D. Cymes and C. Grosman 1 Introduction Ion channels owe their central role in cell physiology to their ability to change co ...

Engineered Ionizable Side Chains 7 It was precisely to overcome the elusiveness of the open state that we developed the single-m ...

8 G. D. Cymes and C. Grosman mation. Of course, because ion currents do not flow through the non-conductive conformations, proto ...

Engineered Ionizable Side Chains 9 This result allowed us to identify the stripe of the M2 α-helix that faces the lumen in the o ...

10 G. D. Cymes and C. Grosman to “lock” the open-channel current at a sublevel even at pH 9.0 (see for example the case of posit ...

Engineered Ionizable Side Chains 11 histidines to reveal the conductance of the main level at positions where substi- tuted lysi ...

12 G. D. Cymes and C. Grosman as that of a lysine at this position. Positions 1ʹ and 10ʹ, also on the sides of M2, dis- played a ...