2250
0nM 10nM
100nM 0.1nM
0nM+H2O2 10nM+H2O2 100nM+H2O2 0.1nM+H2O21nM
1nM+H2O220406080100120140160180Serpinin pGlu-Serpinin*
*Relative Cell Death(Abs 500 nm)Fig. 10 Anti-apoptotic effects of serpinin and pGlu-serpinin. AtT-20 cells were challenged with
50 μM hydrogen peroxide in the presence or absence of 10 and 100 nM serpinin or 0.1 and 1 nM
pGlu-serpinin for 1 day. Cell viability was quantified by the lactate dehydrogenase assay. Serpinin
and pGlu-serpinin significantly inhibited hydrogen peroxide-induced cell death (n = 3, *P < 0.05)
(From Koshimizu et al. 2011b)
Fig. 11 pGlu-serpinin exhibits anti-apoptotic activity. Cultured rat cortical neurons were chal-
lenged with 50 mM hydrogen peroxide (H 2 O 2 ) in the presence or absence of 10 nM pGlu-serpinin
for 1 day. (A) Note the significant reduction in neuronal cell death, as measured by MAP 2 immu-
nostaining, in the presence of H 2 O 2 without pGlu-serpinin treatment (Con.), that was prevented
when the neurons were incubated with pGlu-serpinin (From Koshimizu et al. 2011b). (B) pGlu-
serpinin up-regulated Bcl-2 mRNA expression after H 2 O 2 -induced oxidative stress. Primary E18
rat cortical neurons were cultured for 5 days and then treated with 10 nM pGlu-serpinin or vehicle
for 24 h. Neurons were then treated with 50 μM H 2 O 2 to induce cell death. Twenty-four hours later,
the neurons were collected for qPCR assay to assess the mRNA level of Bcl-2 in different groups.
**p < 0.01 (t-test) (From Loh et al. 2012a)
Serpinin Peptides: Tissue Distribution and Functions