Fig. 4.14 NanoES innervated synthetic cardiac patch. a, bImages showing a-actinin of
cardiomyocytes (green ina–e, Alexa Fluor®488), cell nuclei (blue ina–e, Hoechst 34580) and
PLGAfibers (red inb–d, rhodamine 6G). Dense cardiomyocyte growth was supported by both
nanoES (marked by yellow arrows in (a) and electrospun PLGAfibers in hybrid PLGA/nanoES in
(b).cis a zoomed view of the rectangular box in (b), showing (yellow arrows) striated patterns of
a-actinin (green).dConfocalfluorescence images of synthetic cardiac patch.IIandIIIzoomed-in
view of the upper and lower dashed regions inIshowing metal interconnects, SU-8 scaffold
(arrows inII), electrospun PLGAfibers (arrows inIII).eEpi-fluorescence image of the surface of
the cardiac patch. Green (Alexa Fluor®488):a-actinin, blue (Hoechst 34580): cell nuclei. The
position of the source-drain electrodes is outlined with dashed lines. Scale bar, 200μm(a), 20μm
(b), 40lm(d) and 40lm(e)
4.3 Results and Discussion 55