RNA (contd)
2 -O-methylation (contd)
processing 257–262, 267
translation 273–279
epigenetic phenomena 281
epigenetic phenomena 281–283
formaldehyde reaction 300
guide strand 198
helix
structure & function 283–289
infectious mobile element 268
interference, RNAi 197
nonsense-mediated decay 273
passenger strand 198
poly-A tail 264
polyadenylation 263, 264
primary structure 56, 57
processing 224, 257
quality control
hydration 255
removal 272
short hairpin, shRNAs
ribose zipper 258, 261
‘slippery sequence’, An 198
small nuclear, snRNA
apolipoprotein B 270
small nucleolar RNA, snoRNA
deaminase reactions 270
tertiary structure 269
tertiary structure – metal ions
misfolding 261
translation 259
transversional editing
thermodynamics 63
untranslated regions, UTRs 263
virus 273
RNA analysis in situ
Southern blotting 188
RNA chaperone proteins
RNA folding 261
RNA hairpin
protein recognition 387
RNA helix
average parameters 29–30
RNA mobile element
Alu 281
L1 281
retrotransposons 216, 281
RNA motif
A-minor 257
kissing hairpin complex 257, 258
self-cleaving 285, 286
tetraloop receptor 257, 259
RNA polymerase
transcription inhibition 125
yeast pol IIstructure 411–412
RNA processing 263–272
RNA splicing 264–269
exon omission 211–212
pre-mRNA splicing 265
spliceosome 265–266
RNA structure 55
A-platforms 259, 262
bulges 61
general features 254
hairpin loop 61, 62, 386
internal loop 61
junctions 62
motifs 253–263
P456 261
primary structure 56–57
pseudoknot 63, 288, 289
R17 virus 55-mer 62
secondary structure 57–59
tertiary architecture 261
viral function 287
RNA transcript
RNA processing 263
RNA transport
from nucleus to cytoplasm 280
RNA-DNA duplexes
structure 59–61
RNAi
genetic manipulation by silencing 283
RNA interference 59, 273, 283
RNA-induced silencer complex, RISC
gene silencing 198
RNA-protein particle, RNPs
ribosome 276–277, (front cover)
RNAse H induction
antisense activity 194
Rosenberg, B.
cisplatin 302, 304
Rp/Sp -AT PS
structure & enzymatic use 115
rRNA
gene clusters 213
ribosomal RNA 263, 276Sanger, F.
DNA sequencing 8
sangivamycin
nucleoside analogue, synthesis 96
satellite DNA
repetitious “junk” DNA 216
selection in vitro, SELEX
oligonucleotide selection 198, 199,
200, 202
selenocysteine
UGA code for 21st amino acid 274
semi-conservative replication
description 232–233
Meselson-Stahl experiment 440
sequencing
automated fluorescent 168, 302, 303
DNA 168–170
DNA ladder 168
genome 170
Maxam Gilbert 168, 169, 203
dimethyl sulfate reaction 301hydrazine reaction 298
Sanger 168, 169
shapes of components
nucleoside & nucleotide 20–23
short tandem repeats, STRs
DNA fingrprinting 183
showdomycin
nucleoside analogue 88
sigma factor
prokaryotic transcription 224–226
Signer, R.
nucleic acid research 3
single copy gene 213
single locus probes, SLPs
DNA fingerprinting 182
single nucleotide polymorphisms,
SNPs
DNA fingerprinting 188, 222
single strand binding proteins, SSBs
DNA replication 234
siRNA, small interfering
RNA 283, 421
skin cancer
ozone barrier 316, 321
SN 6999
DNA groove binding 370
snRNA
splicesome component 263
solenoid
chromosome structure 69, 409
Sonogashira reaction
modified base synthesis 93
Southern blot
DNA fractionation 181, 222
Southern, E.
Southern blot 181
specificity
DNA enzymes 404–407
spectroscopic properties
nucleoside & nucleotide 19–20
spine of hydration
DNA structure 30, 346, 347
splicing
optional exon usage 212
stavudine, d4T
antiviral nucleotide analogue 130
steric block oligonucleotide
cDNA block RNA
recognition 193–194
stop codon
premature 273
structural dynamics
DNA motion 64
rapid motions 68
sugar pucker 20
C2-endo29, 386
NMR analysis 435–436
C3-endo20, 29, 386
NMR analysis 435–436
NMR 435468 Subject Index