Cannabis sativa L. - Botany and Biotechnology

(Jacob Rumans) #1
Table 13.1

(continued)

Explant

Response

Medium

Reference

Apical and axillary buds

Shoot, root

MS + 0.45 mg/l BAP + 20 mg/lIBA

Richez-Dumanois et al.(^1986

)

Leaf callus

Shoot

Mandolino and Ranalli(^1999

)

Callus

Root

Mackinnon et al. (

2000

)

Internodes, axillary buds, petioles

Callus, shoot regeneration

MS + 2.0 mg/l and 3.0 mg/ldicamba

Slusarkiewicz-Jarzinaet al. (

2005

)

Roots, leaves, stem

shoot regeneration

DARIA medium

Plawuszewski et al.(^2006

)

Cotyledon, stem root

Callus, shoot regeneration

DARIA medium

Weiglus et al. (

2008

)

Shoot regeneration

Formula

b

based medium

Casano and Grassi (

2009

)

Lateral buds from seedling

Shoot and root regeneration

MS + TDZ + NAAMS + IBA

Bing et al. (

2007

)

Nodal segments with axillary buds

Direct organogenesis; Shoot and root regeneration,synthetic seed

MS + 0.5

μM TDZ

MS + 2.5

μM IBA

Lata et al. (

2009a

,b

,

2012

)

Nodal segments with axillary buds

Direct organogenesis; Shoot and root regeneration MS + 2

μM m-topolin

Lata et al. (

2016

)

Shoot tips

Shoot, root

MS + 0.2 mg/l TDZ + 0.1 mg/lNAAMS+ 0.1 mg/l IBA + 0.05 mg/lNAA

Wang et al. (

2009

)

Epicotyl

Shoot and root regeneration

MS + 2 mg/l BAP + 0.5 mg/l IBA Movahedi et al. (

2015

)

Cotyledons

Shoot and root regeneration

MS + 0.4 mg/l TDZ + 0.5 mg/lIBA

Chaohua et al (

2016

)

MS

Murashige & Skoog medium;

B5

Gamborg medium;

BAP

6-Benzylaminopurine;

DMSO

Di methyl sulphoxide;

2,4-D

2, 4-di chloro phenoxy acetic acid;

2,4,5


  • T


:2,4,

5-tri chloro phenoxy acetic acid;

IBA

Indole 3 butyric acid;

KIN

Kinetin;

NAA

Napthalein acetic acid;

TDZ

Thidiazuron

290 H. Lata et al.

Free download pdf