Precision Medicine, CRISPR, and Genome Engineering Moving from Association to Biology and Therapeutics
56 features, and the complex connections between them. Similar to the advantages seen from the genomics perspective, CRISPR GE c ...
57 in tandem and/or the recruitment of activation domains to modular gRNA scaf- folds have been used (Fig. 3.3a, c) [ 43 – 46 , ...
58 The studies presented thus far have been conducted in cells ex vivo, but a handful of reports have demonstrated the feasibili ...
59 category of GE, we present them here for two reasons: (1) they help to inform on the correlative relationship between genome ...
60 as they bind and diffuse in a live nucleus [ 146 ]. Pairing this type of imaging with the labeling of genomic loci can, for e ...
61 3.5.1 Lineage Tracing with CRISPR GE A key piece of information required to understand the development of multicellular organ ...
62 code containing a series of Cas9 target sites: the loss of information stemming from inter- target deletions, also termed dro ...
63 target. In theory, as long as the PAM is not disrupted, this approach allows for multiple rounds of editing, which can be dec ...
64 expression or activity. This type of tool could potentially be used to create a perma- nent record of cell signaling inputs o ...
65 a process of self- organization—stemming from cell-cell interactions, as well as spatially restricted differentiation [ 163 ] ...
66 human health. The ability to generate healthy tissues and organs from a patient’s own cells will transform the field of medic ...
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70 Corces MR, Buenrostro JD, Wu B, Greenside PG, Chan SM, Koenig JL, et al. Lineage- specific and single-cell chromatin access ...
71 Xu X, Tao Y, Gao X, Zhang L, Li X, Zou W, et al. A CRISPR-based approach for targeted DNA demethylation. Cell Discov. 2016;2 ...
72 Apostolou E, Ferrari F, Walsh RM, Bar-Nur O, Stadtfeld M, Cheloufi S, et al. Genome-wide chromatin interactions of the Nanog ...
73 Sulston JE, Schierenberg E, White JG, Thomson JN. The embryonic cell lineage of the nema- tode Caenorhabditis Elegans. Dev B ...
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© Springer International Publishing AG 2017 75 S.H. Tsang (ed.), Precision Medicine, CRISPR, and Genome Engineering, Advances in ...
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