Quorum Sensing
the transcriptional regulators LasR and RhlR, respectively [1]; in turn, these regulators can alter gene expression of as much a ...
these proteins and activates p38 and p42/44 MAPK cascades, causing the dissociation of junction complexes and compromising barri ...
(Millipore, Temecula, CA, USA). All primary Abs are diluted in blocking buffer. Secondary Abs for Fluorescence Staining: Alexa ...
3 Methods 3.1 Transepithelial Electrical Resistance (TER) and Paracellular Flow TER and paracellular flux is evaluated using a ...
[Ω]) area of the membrane (0.33 cm^2 ) as described by Katouli et al. [16]. Prior to beginning the experiment, cell monolayers ...
then immediately lysed with ice-cold cell lysis buffer (seeSub- heading2). 1 ml cell lysis buffer per 10^7 cells in a 150 cm^2 f ...
Fig. 2 Epithelial tight junctions in 3O-C 12 -HSL-treated human cells. (a) Expression of ZO-3-JAM-A protein complexes in epithel ...
Immunocomplexes are captured by adding 100μl 4 Fast Flow beads and incubating overnight at 4C with gentle rocking on a rocker/ ...
Repeat wash procedure in 1PBS containing 0.18% Tween 20 at least 3–5 times and then visualize the immunoreactive bands. A capa ...
For CLSM, specimens can be examined using any appropriate confocal laser scanning microscope (Fig.2b) and fluorescence staining ...
4 Notes Synthetic 3O-C 12 -HSL and 4 kDa or 10 kDa fluorescein iso- thiocyanate (FITC)-dextrans can be purchased from Sigma Ald ...
Karlsson T, Turkina MV, Yakymenko O, Mag- nusson KE, Vikstrom E (2012) ThePseudomo- nas aeruginosa N-acylhomoserine lactone quo ...
Chapter 18 Animal Models forPseudomonas aeruginosaQuorum Sensing Studies Damien Maura, Arunava Bandyopadhaya, and Laurence G. Ra ...
serious burn injuries result in immunosuppression that predisposes affected patients to opportunistic nosocomial infections. In ...
can be as significant as that of antibiotic resistance. The murine persistent/relapsing full-skin thickness burn injury model ut ...
Shaker incubator set at 37C 200 rpm for bacterial culture growth. 1.5 and 15 ml bacterial culture glass tubes. Spectrophotomet ...
3 Methods 3.1 Burn and Infection Model 3.1.1 Bacterial Inoculum Preparation StreakP. aeruginosaPA14 cells from a 80 C stock o ...
Dilute the bacterial culture 1/1000 in LB-Lennox, then dilute 1/3 serially 20 times in 5 ml LB-Lennox and incubate the tubes at ...
3.1.2 Animal Infection 1. Perform intraperitoneal (i.p.) injection of 500μl of a 6.25 mg/ ml ketamine and 0.625 mg/ml xylazine a ...
agar plates. Count the CFUs after an overnight incubation at 37 C to determine the bacterial inoculum concentration. Assess an ...
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