Quorum Sensing

Cut the tissue samples into small pieces with dissection scissors to facilitate homogenization. Weigh the tissue samples and ad ...

treatment arrest using E-test to confirm that cells are antibiotic tolerant (unchanged MIC) rather than antibiotic resistant (in ...

Assess the bacterial inoculum concentration as described in Subheading3.1.2,step 8. Record animal survival over time (seeNote 1 ...

Depilate with depilatory cream for 1 min (Fig.4e), then wipe the abdomen clean with soft tissue paper to remove any trace of cr ...

Perform all animal infections within 2 h of cell preparation. Make sure to wipe each mouse’s abdomen thoroughly first with a so ...

In this lung acute infection model, an inoculum of 5 106 CFU induces ~50% mortality. Most animals start succumbing infection b ...

Pseudomonas aeruginosa: useful tools for cystic fibrosis studies. Lab Anim 42:389–412 Stevens EJ, Ryan CM, Friedberg JS, Barnhi ...

Chapter 19 Methods to Study Quorum Sensing-Dependent Virulence and Movement of Phytopathogens In Planta Shulamit Manulis-Sasson ...

extracellular enzymes, dispersal of biofilm and other determinants involved in ecological competence [7, 8]. Here, we describe m ...

2.2 Detection and Measurement of DSF The DSF biosensor strainXanthomonas campestrispv.campes- tris8523. Strains to be tested fo ...

Sterile scissor. Sterile razor. Sterile glass microscope slides. Petri dishes. Paraffin film. Fresh plant extract (seeSubheadin ...

Transfer 50μl of theXhpculture (OD 595 ¼0.25–0.3) and slowly pipette the suspension on each disc allowing the suspen- sion to b ...

treatment use 20 plants and the experiments should be repeated three times. Transfer the plants to the greenhouse and inspect t ...

3.6 Determination of Aggregate Formation by Confocal Microscopy Another approach to study the effect of QS on virulence is to de ...

Pipette 50μl of the resuspended culture strains into wells containing the different media. Prepare three to five replicates for ...

we recommend performing preliminary tests to examine the stability of thegfp-plasmid and fluorescence emission of the tested bac ...

He Y-W, Zhang L-H (2008) Quorum sensing and virulence regulation inXanthomonas cam- pestris. FEMS Microbiol Rev 32:842–857 Butt ...

Chapter 20 Differential Equations Models to Study Quorum Sensing Judith Pe ́rez-Vela ́zquez and Burkhard A. Hense { Abstract Mat ...

Models can be used to test a hypothesis of how a system works, to try to estimate how a certain event could affect the system (f ...

1.2 Differential Equations Differential equations (DE) are mathematical tools to study changes. While an equation in general inv ...