Quorum Sensing

INDEX

A

Accessory gene regulator (Agr)..............89–95, 179, 363
Actinorhodin.................................................................118
Acylated homoserine lactone (AHL)
extraction..............................7, 10, 12, 38, 55, 56, 66
hydrolysis........................................................298, 307
N-butanoyl homoserine lactone (C4-HSL).........4, 6,
9, 25, 49, 50, 55, 56, 58, 203, 204, 325, 351
N-decanoyl homoserine lactone (C10-HSL)......... 56,
263, 306
N-hexanoyl homoserine lactone (C6-HSL)....... 4–10,
14, 16–18, 20, 21, 56, 193, 299
N-octanoyl homoserine lactone (C8-HSL)............ 10,
13, 14, 21, 56, 306
N-3-oxo-dodecanoyl homoserine lactone
(3-oxo-C12-HSL)...........................25, 26, 49,
50, 55, 56, 58, 203, 264, 306
N-3-oxo-hexanoyl homoserine lactone
(3-oxo-C6-HSL) ..........................56,211, 306
N-3-oxo-octanoyl homoserine lactone
(3-oxo-C8-HSL) .................................. 56, 306
purification ..............................................................151
receptors...................................................................... 6
synthases............................................. 6, 7, 37, 39, 43,
45, 161–167, 170, 171, 174, 175
Acyl carrier protein (ACP)...................................... 36, 37,
162–168, 170–174
Agar overlay........................................................16, 17, 31
Aggregate formation...........................244, 246, 249, 251
Alginate beads......................................................205–207
Alkaline protease............................................................. 25
Antibiotic
bioassay..........................................118, 121, 123–125
production regulation....................................118, 123
tolerance............................................ 9,228, 230, 234
Anti-quorum sensing
antibodies................................................................326
polyclonal antisera...................................................332
Anti-virulence............................................... 91,228, 288,
334–337, 353
Aromatic substrate specificity assay...................38, 41, 44
Autoinducers (AI)......................................................4, 35,
61, 73–80, 82, 83, 85–87, 131, 146, 150,
161–175, 213–224, 254, 259–270,
353–361

Autoinducer 2 (AI-2)

detection......................................................73–80, 82,

83, 85–87

4,5-dihydroxy-2,3-pentandione (DPD)...........78, 85,

354–356, 359, 361

furanosyl borate diester (BAI-2).......................74, 75,

78, 81–85, 87

quantification.............................73–80, 82, 83, 85–87

(2S,4S)-2-methyl-2,3,3,4-

tetrahydroxytetrahydrofuran-borate............. 74

Autoinducing peptide (AIP)

agr-type autoinducing peptides..........................89–95

detection..............................................................89–95

B

Biofilm

dispersion.........................................3–7, 9, 10, 12–21

flow cell system......................................277, 278, 282

formation.................................................5, 9, 97,108,

131, 145, 213, 249, 275, 278, 282, 288, 297,

325, 353

Bioluminescence......................................... 32, 33, 74, 91,

145, 259, 290, 294, 297, 319, 320, 361

Bioreporter ................................................................27, 30

Biosensor strain.................................................10, 12, 14,

244–246, 290, 292, 294, 300

Boron-doped diamond (BDD) electrode...........107–115

Brominated furanones...................................................... 5

Burkholderia cepaciacomplex (Bcc)............................193

C

Caenorhabditis elegans........................326, 329, 330, 336

Calcium signaling..........................................................224

Cationic surfactant.......................................108, 111–113

(^14) C-carboxy-methionine................................................. 35
cDNA...................................................................177, 178,
185, 186, 190, 338–340
Cell junction..................................................................214
Cholerae autoinducer-1 (CAI-1).................................353
Chromatogram........................................... 18, 52, 53, 69,
70, 98, 102–104, 166, 172, 368
Chromatography...............................................5, 6, 8, 14,
18, 19, 27, 38, 49–58, 61–67, 69–71, 76, 78,
97–102, 104, 105, 118, 195, 350, 370
Coculture assay..............................................................287
Livia Leoni and Giordano Rampioni (eds.),Quorum Sensing: Methods and Protocols, Methods in Molecular Biology,
vol. 1673,https://doi.org/10.1007/978-1-4939-7309-5,©Springer Science+Business Media LLC 2018
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