RNA Detection
LE probe contains two regions: region I on 3^0 -end hybri- dizes with adaptor probe and region II on 5^0 -end has com- plementar ...
Add loading buffer to each of the reactions, mix thoroughly and centrifuge for several seconds. Carefully load DL2000 DNA marke ...
Quickly decant the wells and wash with 300μL of wash buffer for three times, with quick decanting in between. Centrifuge the pl ...
Centrifuge the lyophilized oligonucleotides at 600gfor 2 min and carefully add TE to the tube after centrifuging to dissolve t ...
design offered cooperative hybridization between the capture probes on the solid surface and the multiple CEs binding to one tar ...
Chapter 13 In Situ Detection of MicroRNA Expression with RNAscope Probes Viravuth P. Yin Abstract Elucidating the spatial resolu ...
cytoplasm, these precursor microRNAs are cleaved by the essential enzyme Dicer, into single-stranded functional units [4]. Micro ...
2 Materials and Solutions All solutions are prepared using reverse osmosis water and filter- sterilized prior to use. Aliquots a ...
tissue quickly without promoting crystallization even at the most extreme temperatures. Wash buffer (1PBT): To make 1 L of PBT ...
Hearts are dissected from adult zebrafish using iridectomy scissors and Dumont #5 fine-tip forceps and placed directly into 1.5 ...
Remove fixative solution and replace with 1 mL 30% sucrose. Allow tissues to equilibrate for 2 h to overnight at 4C(see Note 6 ...
Remove slides from Coplin jar and gently tap on a stack of paper towels to remove excess PBS. It is not necessary to completely ...
Remove slides from Coplin jars and gently tap on a stack of paper towels to displace excess liquid. Place slides in your humidi ...
3.5 Signal Detection and Mounting Detection of amplified in situ signal is dependent on the desired chemistry and probe design. ...
Remove slides and immediately coverslip with 3–5 drops of EcoMount mounting medium. Be cautious to avoid trapping air bubbles, ...
Fixation can alter protein biochemistry such that the epitope of interest becomes masked and is no longer assessable by the pro ...
Giraldez AJ, Cinalli RM, Glasner ME, Enright AJ, Thomson JM, Baskerville S, Hammond SM, Bartel DP, Schier AF (2005) MicroRNAs r ...
Chapter 14 Padlock Probes to Detect Single Nucleotide Polymorphisms Tomasz Krzywkowski and Mats Nilsson Abstract Rapid developme ...
quantitation and localization of mRNAs feasible. Although various FISH approaches facilitate assessment of mRNA levels in indivi ...
oligonucleotides), complementary to the motif repeated in the amplicon. Local concentration of decorator probes creates a bright ...
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