RNA Detection

that heterogeneities in cell morphometry or microenvironment are the dominant source of cell-to-cell variability in this system. ...

in controlling RNA fate [79]. The discovery of the prevalence of RNA localization raises the question of its global functional i ...

probes that only fluoresce upon association with target RNAs is another strategy adopted to minimize background signals due to u ...

3.1.3 Engineering Fluorescent Proteins for Recognition of Endogenous RNAs To overcome the limits of monitoring genetically modif ...

Dual color imaging has also been key in dynamically studying nucleocytoplasmic export, a poorly studied yet active and selective ...

A complex regulation of molecular motors has been described in different studies [104, 125–128], and is responsible for directed ...

distal region of dendrites [118, 130]. Interestingly, and opposite to the previous assumption that mRNAs are transported in a si ...

posttranscriptional regulatory networks, these methods require a large quantity of material and are not well-adapted to map RNA– ...

spliceosome and ribonucleoprotein dynamics, raising the intriguing possibility that RNAs might control many cellular processes b ...

role in this process by promoting the establishment of multivalent interactions. Highlighting the need for dynamic interactions ...

3D environment with high sensitivity and temporal resolution. In this respect, recently developed super-resolution microscopy te ...

posttranscriptional gene regulation. Curr Opin Chem Biol 30:46–51. doi:10.1016/j. cbpa.2015.10.024 Dominissini D, Nachtergaele ...

161(5):1202–1214. doi:10.1016/j.cell. 2015.05.002 Jaitin DA, Kenigsberg E, Keren-Shaul H, Ele- fant N, Paul F, Zaretsky I, Mild ...

Single-molecule RNA detection at depth by hybridization chain reaction and tissue hydro- gel embedding and clearing. Development ...

tissue and cells. Nat Methods 10(9):857–860. doi:10.1038/nmeth.2563 Lee JH, Daugharthy ER, Scheiman J, Kalhor R, Yang JL, Ferra ...

interactions in situ using modified-MTRIPs and proximity ligation. Nucleic Acids Res 41 (1):e12. doi:10.1093/nar/gks837 Tyagi S ...

encoded fluorescent probes. Anal Chem 83 (14):5708–5714. doi:10.1021/ac2009405 Yoshimura H, Inaguma A, Yamada T, Ozawa T (2012) ...

Halstead JM, Lionnet T, Wilbertz JH, Wip- pich F, Ephrussi A, Singer RH, Chao JA (2015) Translation. An RNA biosensor for imagi ...

Castello A, Fischer B, Frese CK, Horos R, Alleaume AM, Foehr S, Curk T, Krijgsveld J, Hentze MW (2016) Comprehensive identifi- ...

Wheeler JR, Matheny T, Jain S, Abrisch R, Parker R (2016) Distinct stages in stress gran- ule assembly and disassembly. Elife 5 ...