- Sodium deoxycholate.
- Urea.
- EDTA pH 8.0.
- MgCl 2.
- Tween 20.
- Dithiothreitol.
- PEG400.
- LDS-4sample buffer.
- Methanol.
- 20MOPS-SDS running buffer.
- 20transfer buffer.
- Neutral phenol–chloroform.
- TE buffer pH 7.0.
- TE buffer pH 8.0.
- 3 M sodium acetate, pH 5.5.
- 80% and 100% ethanol (molecular biology grade).
- 1 M NaOH.
- HEPES pH 7.3.
- 2TBE-UREA loading buffer.
- TBE buffer.
- Magnetic protein G/A beads.
- RBP antibodies.
- Anti-hnRNP C (positive control) (e.g., Santa Cruz sc-32308).
- Protease inhibitor cocktail.
- Anti-RNase antibody (e.g., Thermo Fisher AM 2690).
- RNase I (e.g., Thermo Fisher).
- Turbo DNase (e.g., Thermo Fisher).
- T4 PNK (e.g., NEB).
- RNase inhibitor (e.g., Promega RNasin).
- T4 RNA ligase I.
- Near infrared protein marker (e.g., Li-Cor Chameleon).
- Antioxidant (e.g., Life Technologies, NP0005).
- Reducing agent (e.g., Life Technologies, NP0004).
- Proteinase K (e.g., NEB).
- GlycoBlue.
- 10 mM dNTPs.
- Reverse transcriptase (e.g., ThermoFisher Superscript III).
434 Christopher R. Sibley