Endophytes Crop Productivity and Protection Volume 2 (Sustainable Development and Biodiversity)

Table 8.1

(continued)

Crop

Planttissue

Sterilization methodemployed

Strategy of planttissue inoculation

Isolation medium

Antimicrobialsupplementedto growthmedium

Number ofrecoveredendophyticactinobacterialisolates

Identi

fication of

endophyticactinobacterial isolates

References

Citrus rootstocks(several citruscultivars)

Leavesand seeds

Rinsed with ethanol70%Sodiumhypochlorite (3%)for 3 minRinsed once inethanol 70%Rinsed twice insterile distilledwater

Leaveshomogenized in ablender with 5 mLof sterile NaCl0.85% and seriallydilutedLeaf and seed cutinto 4

• 6mm

fragments

Trypticase soy agar(TSA)

Benomyl(50

lg/mL)

N.i.

Curtobacteriumflaccumfaciens

Ara

újo et al.

(^2001

)

Sweet-orange(Citrus sinensisOsbeck cv.Natal)Tangerine (

C.

reticulata

cv.

Blanco)

Branches Ethanol 70% for

5 minSodiumhypochlorite (2%)for 5 minEthanol 70% for30 sTwo rinses in steriledistilled waterBark ofsurface-disinfectedbranches removedwith a sterilizedrazor blade

Bark ofsurface-disinfectedbranches removedBranches cut into 4 –

6 mm fragments

TSA

Benomyl(50

lg/mL)

N.i.

CurtobacteriumflaccumfaciensNocardia

spp.

Streptomyces

spp.

Ara

újo et al.

(^2002

)

(continued)

8 Endophytic Actinobacteria for Sustainable Agricultural Applications 169

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