Caspases,Paracaspases, and Metacaspases Methods and Protocols

Caspases, Paracaspases, and Metacaspases Peter V. Bozhkov Guy Salvesen Editors Methods and Protocols Methods in Molecular Biolog ...

M ETHODS IN MOLECULAR BIOLOGY Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hat fi eld, Hertf ...

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Caspases, Paracaspases, and Metacaspases Methods and Protocols Edited by Peter V. Bozhkov Department of Plant Biology, Uppsala B ...

ISSN 1064-3745 ISSN 1940-6029 (electronic) ISBN 978-1-4939-0356-6 ISBN 978-1-4939-0357-3 (eBook) DOI 10.1007/978-1-4939-0357- Sp ...

v Among a plethora of known proteases, caspases are perhaps the ones that have attracted and continue to attract much more resea ...

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vii Contents Preface.......................................................... v Contributors................................... ...

viii 13 Purification, Characterization, and Crystallization of Trypanosoma Metacaspases....................................... 2 ...

ix A NDREAS BERGMANN • Department of Cancer Biology , University of Massachusetts Medical School , Worcester , MA , USA D AVE BO ...

x G UR P. KAUSHAL • Central Arkansas Veterans Healthcare System , Little Rock , AR , USA ; Department of Internal Medicine , Uni ...

xi M ARGOT THOME • Department of Biochemistry , University of Lausanne , Lausanne , Switzerland L IANA TSIATSIANI • Biomolecular ...

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Part I Caspases ...

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3 Caspases, Paracaspases, and Metacaspases: Methods and ProtocolsPeter V. Bozhkov and Guy Salvesen (eds.), , Methods in Molecula ...

4 biochemical studies, these methods have populated a list of more than 1,400 caspase substrates [ 11 , 12 ]. In most circumstan ...

5 occur in E. coli, and caspases do not require any posttranslational modification to display full activity. Consequently, it is ...

6 DTT (freshly added), and 2 mM ethylenediaminetetraacetic acid (EDTA) (filter-sterilized). Eukaryote lysis buffer: 50 mM HEPES ...

7 Washing buffer: 50 mM Tris at pH 8.0, and 0.5 M NaCl (autoclave-sterilized). 2× TY media (1 L): 16 g tryptone, 10 g Bacto yea ...