263Peter V. Bozhkov and Guy Salvesen (eds.), Caspases, Paracaspases, and Metacaspases: Methods and Protocols,
Methods in Molecular Biology, vol. 1133, DOI 10.1007/978-1-4939-0357-3, © Springer Science+Business Media New York 2014
A
Ac-LRSR-AMC ...................................... 179, 181, 183, 186
Active-site titration .................................... 1 0, 17–21, 25, 31
Ac-VRPR-AMC ....................... 1 91, 192, 194, 200, 240, 245
Affinity labeling ................................................ 7 8, 82–83, 85
Amex method ............................................................... 9 7, 99
7-amino-4-carbamoylmethylcoumarin
(ACC) ....................................... 4 2, 44–46, 48–57
Aminoluciferin .................................................................1 28
7-amino-4-methyl-coumarin (AMC) ................... 4 3, 44, 56,
76, 79, 80, 103–105, 142, 145, 146, 159, 163, 168,
179, 181, 183, 186, 191, 192, 194, 198, 200, 208,
214, 215, 219, 240, 245–247, 251
7-amino-4-trifluoromethylcoumarin
(AFC) .........................................5 , 11, 15–17, 21,
24, 25, 28, 31, 32, 44, 56, 76, 79, 80, 91, 93, 95,
99, 142, 145, 146, 159
Anion exchange chromatography ............................. 4 , 11, 12
Antigen retrieval ....................................... 1 14, 144, 149, 152
Apoptosis ....................................................2 6, 33, 41, 72–74,
101, 109, 119–121, 123, 124, 126–128,
130–135, 137–139, 151, 158–160, 164, 166–169,
172, 173
Apoptosome ................................................. 1 9, 91, 130, 146
Autoprocessing ................................... 9 5, 157, 238, 242, 245,
247, 248, 251
Autoradiography ............................ 7 4, 81, 129, 148, 249, 250
B
Baculoviral protein p35 .......................................................33
B-cell lymphoma ..............................................................1 77
BCL-2 ....................................... 119, 123, 131–133, 158, 159
Bcl10 ................................................................ 178–182, 184
BCL-xL ........................................................... 1 23, 132–133
Beer-Lambert law ............................................................2 20
Biotin-VAD-fmk .........................................7 4, 78, 82, 83, 85
Bloomington Drosophila Stock Center .................... 1 11, 113
BL21 strain ................................................7 , 28, 92, 102, 219
Bovine serum albumin (BSA) .............................. 7 8, 82, 144,
145, 152, 161–163, 169, 183, 194,
197, 198, 201
Breast cancer carcinoma MCF-7 cells ................................2 3
Butyrylation ......................................................................2 56
C
Calcium ....................................................1 25, 137, 184, 204,
205, 213, 245–248, 251
CASBAH/MerCASBA databases ....................................1 69
Caspase-1 .................................. 25, 26, 43, 99, 142, 145, 163
Caspase-2 ................................... 8, 15, 19, 24–25, 29, 32, 33,
71–85, 120, 122–123, 129–133, 138, 142, 145,
151, 156, 161, 163, 171
Caspase-3 ................................... 8, 15, 17, 19, 22, 23, 25–27,
29, 32–34, 72–74, 110–113, 119, 120, 122–124,
127–129, 131–133, 135–136, 138, 142, 145, 146,
148, 151, 152, 156–159, 161–163
Caspase-4 ......................................................... 242, 249, 250
Caspase-6 .............................................19, 25, 28, 29, 31–34,
142, 145, 146, 151, 158, 161–163
Caspase-7 .......................................................... 8, 18, 19, 22,
23, 25–27, 29, 32, 33, 37, 120, 122–124,
127–129, 132, 133, 135–136, 138, 142, 146, 151,
156, 158, 161, 163
Caspase-8 ................................. 4, 7, 8, 10–13, 19, 23, 25–27,
29, 31–33, 36, 129, 131, 142, 145, 146, 151, 152,
156, 158, 161, 163
Caspase-9 ......................................... 8, 19, 26, 27, 29, 32, 33,
129, 130, 142, 145, 146, 151, 152, 158, 161–163
Caspase-10 .................................... 8, 19, 26, 32, 33, 146, 151
Caspase-14 ............................................................. 44, 89–99
Caspase(s)
in Caenorhabditis elegans ...................................... 101–107
in Drosophila ....................................................... 109–115
in mammalian cell cultures ................................. 1 55–173
in mice ................................................................ 1 41–153
in Xenopus laevis ................................................. 119–139
Caspase activation recruitment domain
(CARD) ................................................. 8 , 19, 26,
29, 71, 72, 84, 156
Caspase substrates ........................................4 , 15, 20–24, 30,
36, 41–58, 61–70, 77, 83, 91, 103, 121, 122, 124,
127–129, 135, 142, 146, 157–158, 162, 163,
167–169, 238, 246
Catalytic constant ...............................................................1 4
Catalytic dyad .............................. 2 6, 189, 190, 203, 204, 223
Catalytic mutant ............................................................. 8 , 26
Catalytic specificity .............................................................1 5
CED-3 ..................................................................... 101–107