AMPK Methods and Protocols

(Rick Simeone) #1

  1. Xiao B, Sanders MJ, Carmena D, Bright NJ,
    Haire LF, Underwood E, Patel BR, Heath RB,
    Walker PA, Hallen S, Giordanetto F, Martin
    SR, Carling D, Gamblin SJ (2013) Structural
    basis of AMPK regulation by small molecule
    activators. Nat Commun 4:3017.https://doi.
    org/10.1038/ncomms4017

  2. Hunter RW, Foretz M, Bultot L, Fullerton
    MD, Deak M, Ross FA, Hawley SA,
    Shpiro N, Viollet B, Barron D, Kemp BE,
    Steinberg GR, Hardie DG, Sakamoto K
    (2014) Mechanism of action of compound-
    13: an alpha1-selective small molecule activator
    of AMPK. Chem Biol 21(7):866–879.https://
    doi.org/10.1016/j.chembiol.2014.05.014
    24. Scott JW, Galic S, Graham KL, Foitzik R, Ling
    NX, Dite TA, Issa SM, Langendorf CG, Weng
    QP, Thomas HE, Kay TW, Birnberg NC,
    Steinberg GR, Kemp BE, Oakhill JS (2015)
    Inhibition of AMP-activated protein kinase at
    the allosteric drug-binding site promotes islet
    insulin release. Chem Biol 22(6):705–711.
    https://doi.org/10.1016/j.chembiol.2015.
    05.011
    25. Zhou G, Myers R, Li Y, Chen Y, Shen X,
    Fenyk-Melody J, Wu M, Ventre J, Doebber T,
    Fujii N, Musi N, Hirshman MF, Goodyear LJ,
    Moller DE (2001) Role of AMP-activated pro-
    tein kinase in mechanism of metformin action.
    J Clin Invest 108(8):1167–1174.https://doi.
    org/10.1172/JCI13505


Seahorse Analysis of AMPK-Regulated Metabolic Fluxes 305
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