AMPK Methods and Protocols

Carbohydrate.....................................................16, 17, 57,
70, 87–97, 239, 240, 258, 295, 363, 367, 396
Carbohydrate binding module (CBM).................. 16, 17,
70, 87–97, 239, 240, 295
Cardiac conduction disease........................590, 593, 600
Cardiac hypertrophy...........................................321–341,
590, 594, 599, 601, 606, 607
Cardiomyopathy..................................................581, 582,
584, 590, 592, 594, 599, 602–604, 606, 607,
611, 612
Cardiovascular disease (CVD)..............15,159, 508, 510
CD36...................................................348, 353, 355, 356
Cells ......................................................................... 82, 83,
172, 174, 181–183, 186, 263, 295, 310–313,
315, 318, 375, 378–382, 387, 520, 597
bacterial.......................................................... 6, 30, 32,
36, 47, 70–72, 74–76, 82–84, 100, 145, 160,
161, 171, 401, 566
insect............................................................17, 19, 22,
30, 32, 33, 36, 37, 47, 82, 100, 160
mammalian cell lines
Caco-2............................172, 174, 181–183, 186
COS ................................................................... 597
hela........................................................82, 83, 263
NIH3T3.........................375, 378–380, 382, 387
U2OS.......................................375, 378, 380, 381
U937........................................310, 311, 315, 318
mammalian primary cells
human umbilical vein endothelial
cells (HUVEC)..................310–313, 318, 520
mouse bone marrow derived macrophages..... 555
mouse hepatocytes...................................364, 365
mouse lymphocytes...................................465–475
mouse neurons.................................................. 295
rat neonatal cardiomyocytes............324, 327, 356
yeast ...........................................................47, 59,144,
146–148, 150, 154, 155, 174, 597
Cell counting.......................................................235, 324,
330, 333, 466, 467, 470, 471, 474, 545, 561
Cell metabolism..................................172, 298, 303, 414
Cholesterol
homeostasis ...................................477–486, 488–492
reverse transport...................478, 483, 488, 489, 492
Chromatin............................................................565–567
c-Jun N-terminal kinase (JNK)..........307, 308, 312, 317
Clark electrode.....................................................274, 278
Colorimetric assay............................................60–63, 435
Compartmentalization................................259, 261, 270
Compound C............................195–200, 260, 295, 354,
356, 357, 414, 440, 552, 553, 558, 561
Conditioned medium.........................................311, 314,
318, 479, 480, 483, 484, 490
Consensus sequence
mapping............................................................ 99, 100

CPMG relaxation dispersion................ 88,90–93, 95–98

CRISPR/Cas9.....................................171–193, 240, 356

Crystallisation...............................................................1–9,

11–13, 15–22, 24–26, 34, 38, 51, 52

Cytokine....................307–309, 311, 312, 314–317, 521

Cytometry .....................................................................560

D

Dephosphorylation

Thr172............69–71, 74, 81, 82, 84, 230, 255, 417

Detergent-resistant membrane (DRM)......................394,

395, 397, 401, 402, 404, 408, 409

Deuterium exchange,seeHydrogen/deuterium exchange

Differentiation......................................................... 34, 41,

42, 91, 138, 196, 256, 282, 295, 308, 340,

367, 374, 379, 465, 466, 478, 484, 486, 488,

490, 520, 539, 541, 545, 546, 551, 555, 556,

559, 610

Dihydroethidium staining (DHE)..............................496,

498, 501–503, 510, 512, 513, 515, 516

Dorsomorphin,seeCompound C

E

Electron paramagnetic resonance (EPR)

spectroscopy...............................495, 497, 499

Endothelium

function.......................308, 495, 496, 507, 519, 609

Energy

charge.....................................................235, 246, 274

metabolism, cancer,seeCancer metabolism

Enzymology.................................................................... 30

Epigenetics .................................565, 572–574, 577, 608

Eukaryotic elongation factor-2 (eEF2).......................257,

322, 323, 325, 331, 332, 340

Evolution....................................111–140, 195, 229, 565

Expression ..........................................................17, 30, 35

bacterial

tricistronic............................................... 17, 30, 35

mammalian..................................................15, 30, 47,

70, 78, 143, 160, 565, 597

Extracellular flux analyser...................................244, 248,

552, 555, 559.See alsoSeahorse

Extracellular signal-regulated protein kinases 1 and

2 (ERK1/2)..............................199, 322, 323,

328, 337, 339, 341

F

Fatty acid

synthesis................................................273, 279, 363,

368, 373, 423, 434

Feature architecture............................................112, 113,

115, 121, 124–126, 139

Fertility defects..............................................................570

622
AMPK:METHODSANDPROTOCOLS
Index