AMPK Methods and Protocols

Flow cytometry...................................465–475, 521, 560
Fluorescence-activated cell sorting (FACS)................175,
182, 191, 540, 543, 548, 552, 553
Fo ̈rster resonance energy transfer (FRET).................256,
259, 261, 262, 264, 266, 267, 269, 270
Functional equivalence.................................................112

G

Glucose
deprivation...................373, 381, 387, 413, 437, 465
uptake..............................................59,244, 343, 592
GLUT4................................................348, 353–356, 558
Glycogen....................................................................60–62
assay
colorimetric...................................................60–62
fluorimetric....................................................60–62
determination
development enzyme mix.............................60, 61
hydrolysis enzyme mix..................................60–62
storage......................................... 3, 57,594, 607, 610

H

Hanks buffer........................................323, 452, 461, 521
Heart failure .................................................................508,
582, 584, 593, 600, 605, 610, 611
Hepatocytes
isolation.........................................274, 278, 279, 284
Heterotrimer...............................................15–22, 24–26,
31, 36–39, 47, 80, 100, 143, 159–169, 204,
215–218, 220–228, 355, 393, 433, 478
High-performance liquid chromatography
(HPLC).................................................. 49, 50,
230, 231, 233–235, 241, 246, 398, 496, 497,
500, 501, 508, 510, 511, 513–516
Homeostasis ................................................. 30,195, 229,
255, 273, 274, 289, 290, 373, 413, 434, 465,
477–486, 488–492, 507, 509
Homogenization.................................................204–208,
216, 224, 276, 282, 285, 399, 462
Hydrogen/deuterium exchange (HDX)............... 30, 31,
33, 34, 36, 38–42, 47–51
Hypothalamus......................................................433–446

I

Immuno-blot.......................................................152, 155,
161, 164, 186, 191, 193, 325, 332, 333, 339,
374–376, 379–382, 384, 387, 388, 401,
403–405, 407, 408, 422, 450, 462, 554, 561.
See alsoWestern blot
Immuno-precipitation (IP).................................... 70, 73,
74, 78–82, 84, 203–209, 211, 212, 215–218,
220, 221, 224, 226, 240, 249, 394, 397, 401,
402

Immuno-staining ................................................326, 327,

333, 335, 337, 380, 524, 531, 536, 572, 573,

576, 577

Inflammation............................................v,307–318, 478

In silico............................................................99,100, 587

Insulin

secretion.......................414, 415, 417, 421, 425, 609

In vitro........................................................................9, 11,

59, 106, 196–198, 216, 295, 347, 356, 394,

395, 397, 401, 404, 414, 415, 420, 427, 501,

508, 519, 520, 540, 545, 551, 558, 559, 586,

594, 597

In vivo..........................................................v, 30, 43,160,

196–199, 216, 393, 414, 428, 478, 483, 488,

489, 492, 500, 508, 519–521, 536, 552, 557,

558, 586, 588, 597

Islets

human............................................................290, 414,

415, 417, 421, 422, 427–429, 609

isolation..............290, 415, 416, 419, 421, 422, 427

mouse...................414–417, 419–421, 427–429, 609

Isoform................................................................1, 15, 45,

52, 70, 87, 143, 159, 160, 162, 166, 176, 190,

192, 195, 203, 204, 206, 208, 209, 211, 212,

216, 217, 224, 252, 255, 257, 355, 414, 440,

473, 520, 597.See alsoAntibody isoform-

specificty

Isothermal titration calorimetry (ITC).......88, 90,93–97

K

Ketone bodies..............................................290, 301, 303

Ki67 ...................................539, 540, 542, 545–548, 596

Kidney

harvesting .......................................................454, 455

perfusion.........................................................454, 455

slice prepapartion..................................454, 455, 458

Kinase.................................................................70, 80, 82,

100, 216, 223, 227, 241, 249, 256, 258, 261,

421, 440

activity

assay..................... 70, 80, 82,216, 223, 227, 249

monitoring................................................241, 258

inhibitor..........................................................200, 356

dominant-negative mutant......................421, 440

peptide............................................................... 261

small molecule,seeCompound C)

signalling

dynamics ............................................................ 256

target

validation ........................................................... 100

Kinetics..............................................................31, 32, 38,

45, 47, 48, 50, 87–97, 344–347, 425, 466,

491

AMPK:METHODSANDPROTOCOLS

Index^623