Phenformin......................................................83, 84,239,
241–244, 246, 250, 252, 323, 324, 326, 327,
329, 332, 335, 337, 339, 353, 481
Phosphorylation............................................................273
oxidative,seeOxidative phosphorylation
target protein.................................106, 108, 192, 215
Thr172......................................................... 17,69–71,
74, 76, 77, 80, 81, 83, 84, 229, 239, 241, 243,
245, 249, 250, 266, 355, 386, 450, 552, 553,
599
Phylogenetic profile............................................111–113,
116, 119, 123–125, 128, 129
Plasmid DNA.......................................................... 5,174,
176, 179, 243, 261, 263
Polystyrene beads................................466, 470–472, 474
Pre-excitation......................................................582, 584,
585, 587, 588, 590, 592–594, 596, 598, 603,
604, 606, 609, 611
Prey plasmid ...............................144, 147, 149–153, 155
PRKAA1 gene.............69,121, 126–129, 172, 176–178
PRKAA2 gene..............................69,172, 176, 177, 192
PRKAG2 gene....................................................... 69,129,
582, 584, 590, 593, 594, 596–598, 602–607,
609–612
Protein...........................................................................582
extraction.......................................................192, 205,
232, 251, 396, 421, 435, 441
interaction...........................................................29, 32,
88, 93, 111, 143–156, 204, 211, 226, 256
kinase,seeKinase
phosphatase ........................................................70, 74,
82, 211, 226, 230, 258, 397, 433, 446, 554
synthesis..........................................................321, 354
Purification .......................................................... 1, 4,7–9,
12, 16, 18, 19, 21, 22, 35, 37, 38, 48, 51, 53,
70–72, 75, 76, 144, 162, 164, 165, 168, 192,
215, 324, 329, 401, 408, 428, 466
Q
Quantification...............................................................417
adenine nucleotides,seeAdenine nucleotides
quantification
glycogen,seeGlycogen determination
kinase activity,seeKinase assay
Quiescence.....................................................................539
R
Radioactivity
(^14) C ................................332, 345, 349–352, 364–369
(^3) H .........................................................345, 346, 349,
350, 352, 480, 482–484, 486, 488, 490, 491
(^32) P........................................... 78,106, 224, 227, 423
(^33) P..................... 31, 35, 45,218, 222, 224, 226–228
tracer.....................................223, 345, 346, 364, 369
Ragulator.............................................393–399, 401–409
Reactive oxygen species (ROS) ..........................353, 434,
496, 500, 501, 507–517
Recombinant protein........................................30, 32, 35,
37, 47, 105, 160, 246
Respiratory rate.................279, 282, 284, 285, 454, 455
Ribosomal protein S6 kinase beta-1
(p70S6K)...........................................322, 323,
325, 326, 331, 332, 340
Ringer buffer.....................425, 452, 453, 455, 461, 462
R-programming..................................................... 99, 106
S
Seahorse......................................................244, 248, 252,
274, 275, 279–281, 289–303
Signalling,seeKinase Signalling
Sirtuin-1 (SIRT1) ...............................552, 553, 558, 561
Skeletal muscle
regeneration.............................................................539
Specificity......................................................................... 70
antibodies,seeAntibodies
organelle ................................................260, 265, 394
Spheroid
assay.......................................................520, 521, 525
generation..............................................521, 525, 531
Sprouting.............................................................520, 521,
523, 527, 528, 533–535
Stem cell
behaviour.................................................................540
muscle (MuSC) ......................................196, 539–548
Sudden death.......................................582, 590, 600, 606
Surface plasmon resonance (SPR).......................... 30, 32,
35, 39, 46–48, 53, 87
Synthesis......................................................274, 363, 364
fatty acid,seeFatty acid synthesis
lipid,seeLipid synthesis
protein,seeProtein synthesis
sterol,seeSterol synthesis
Systems biology............................................................... 99
T
Target validation,seeKinase target validation
Tissue.......................................................... 15, 58, 59, 64,
65, 70, 73, 151, 163, 203–208, 215, 218,
229–235, 242, 248, 252, 274, 275, 279, 285,
290, 313, 317, 351, 355, 357, 375, 378, 394,
396, 399, 401, 408, 409, 413, 427, 434, 441,
445, 449–453, 455–460, 462, 478–482, 485,
490, 499, 500, 502, 503, 508, 510, 511,
513–516, 524, 529, 532, 536, 539, 557, 560,
573, 576, 592, 597, 599, 607, 610
Transfection........................................................... 30,160,
162, 163, 166, 168, 169, 174, 176, 180, 240,
243, 246, 261–263, 266, 268, 270, 308, 376,
AMPK:METHODSANDPROTOCOLS
Index^625