Esophageal Adenocarcinoma Methods and Protocols

88

6. Recording the location of blocks taken on a specimen photo-
   graph or diagram is recommended.


7. Pack the remaining specimen in such a way that it can be easily
   reconstructed if further blocks are required (see Note 11).


8. Present the findings after microscopic examination (see Note 12).

4 Notes

1. Appropriate personal protection and dissection equipment is
   available in all accredited tissue pathology laboratories. The
   macroscopic assessment forms part of the pathology report
   and is generally transcribed from a dictation recorded during
   the assessment and cut up.


2. Inspect and palpate the specimen to identify the likely site of
   the lesion before opening. The request form and preoperative
   imaging may also provide useful information on tumor loca-
   tion and extent. If the tumor occupies the entire circumference
   of the esophageal mucosa, open the esophagus longitudinally
   from both ends to the edge of the tumor and insert a wick of
   formalin-soaked paper towel before submerging the specimen
   in formalin. If the tumor is non-circumferential, open the

Fig. 2 Example of blocking and sectioning and numbering

Benjamin M. Allanson and M. Priyanthi Kumarasinghe