Basic Concepts in Clinical Biochemistry-A Practical Guide.7z

3.Blue waste bin bag–All broken glass bottles and articles, surgical blades, glass syringes, needles, and any sharp material. 4. ...

Blood Collection and Preservation 2 2.1 Blood Collection Blood is a bodyfluid containing plasma, red blood cells (RBCs), white b ...

Plasma Separating Tubes These tubes contain ethylenediaminetetraacetic acid (EDTA) which is a strong anticoagulant. The tubes sh ...

Anticoagulants Whole blood or plasma sample investigations need use of anticoagulants while collecting sample. Some common antic ...

Quality Control in Laboratory 3 The ultimate goal of the clinical biochemistry laboratory is to analyze the substances in bodyfl ...

While performing analytical measurements in laboratory, various types of errors may be encountered. 3.1 Types of Laboratory Erro ...

calibrators that have expired, sampling errors, and changes in analyzer’s measuring unit.Post-analytical errorsarise during tran ...

If a test is conducted on patient the second time, then the results obtained from such test may be compared with the previous r ...

Automation in Clinical Laboratory 4 Automation is the use of various control systems for operating equipments and other applicat ...

sample. These are usually meant to measure one analyte at a time, but many samples may be measured rapidly. Batch Analyzer In ba ...

separation of serum are performed to enable sample preparation. The use of whole blood for sample analysis and automation of spe ...

Photometry: Colorimeter and Spectrophotometer^5 Photometry deals with the study of phenomenon of light absorption by molecules i ...

Absorbance¼ 2 log 10 T ¼ 2 log%T 5.1 Colorimeter and Spectrophotometer Colorimeter and spectrophotometer work on principle of ...

wavelengths in spectrophotometer even at every 5 or 10 nm ranges. It gives a data spectrum instead of giving a specific absorban ...

The sample cuvette used in spectrophotometer is made of quartz instead of glass cuvette as in colorimeter. Although glass cuvett ...

Preparation of General Laboratory Solutions and Buffers^6 Almost all the experimental techniques in clinical biochemistry requir ...

Molarity MðÞ¼ Number of moles of solute Litre of solution ¼ Weight in gram 1000 Molecular weightvolume mlðÞ For example, the m ...

6.2.1 Normality (N) Normality can be defined as the number of equivalents of a solute dissolved in 1 litre of solution. An equiv ...

6.3 Percent (%) Solutions The % solutions can be expressed as below: (i) Weight/volume (%, w/v): It is the weight of a solute in ...

6.5 Physiological Buffers in the Human Body................ In thefirst instance, pH in the human body is maintained by physiolo ...