Basic Concepts in Clinical Biochemistry-A Practical Guide.7z

contents for 5 min at room temperature. Read absorbance at 640 nm. Calculate protein amount in CSF sample as given for pyrogallo ...

To Measure Lipid Profile in Serum Sample 35 Lipids are organic substances relatively insoluble in water but soluble in organic s ...

4-aminophenazone to form red quinone imine dye. The color produced is measured at 520 nm and compared with that of cholesterol s ...

35.3 Triglycerides Estimation Triglycerides are esters of glycerol possessing usually three different fatty acids. The widely di ...

3.Working triglyceride reagent Add 250μl of enzyme reagent to 30 ml of buffer solution. Mix gently, and keep the reagent for at ...

35.4.2 Reagents 1.Precipitating reagent: This contains 0.55 mM phosphotungstic acid and 25 mM magnesium chloride. 2.Cholesterol ...

calculated on the basis of difference between total cholesterol levels of serum and the cholesterol levels in the supernatant af ...

35.6.2 LDL Values LDL cholesterol below 100 mg/dl is desirable. LDL greater than 150 mg/dl indicates increased risk to cardiovas ...

To Estimate Sodium and Potassium in Serum by Using Flame Photometer^36 36.1 Theory Sodium is the major cation of extracellularfl ...

36.3 Principle........................................ “The test solution is passed asfine spray into theflame. Theflame evapora ...

3.Working standards:Mixed working standards for sodium and potassium are prepared as follows: (a) Sodium/potassium (110/2 mEq/L) ...

When the plasma potassium concentration exceeds 5.5 mmol/L, the condition is called hyperkalemia. Higher potassium levels cause ...

To Perform Radioimmunoassay 37 Radioimmunoassay (RIA) is a highly sensitive and specific analytical tool used for detection of a ...

37.2 Advantage Advantage of RIA is its high sensitivity. It can measure up to few pictograms of antigens. The specificity of the ...

To Perform Enzyme-Linked Immunosorbent Assay^38 Enzyme-linked immunosorbent assay (ELISA) is an immunoassay that combines the sp ...

with enzyme is added. The substrate specific to enzyme is added to well, and colored product formed is measured. The sandwich EL ...

38.4 Direct ELISA In direct ELISA, the antigen is immobilized on the microwell plate. Then an enzyme-labelled primary antibody i ...

Some Important Case Studies 39 39.1 Case Studies of Sugar Impairment..................... Q.1. A diabetic patient on insulin the ...

Q.4. A 45-year-old man was hospitalized with complain of dizziness and weak- ness. His random blood sugar was 40 mg. History rev ...

Glucose: 375 mg/dl Urea: 85 mg/dl Creatinine: 2.1 mg/dl Sodium: 135 mmol/L Potassium: 5.6 mmol/L Chloride: 95 mmol/L Urine was p ...