Synthetic Biology Parts, Devices and Applications

Index 405

genetic engineering, T‐cell therapeutic
function 357
genome reduction
comparative genomics 64
gene essentiality 66
MG1655 68
MGF‐01 67
streamlined‐genome strains 67
genome transplantation 83, 85
Gibson assembly method 335
acetate utilization 335
BglBrick‐style cloning 337
gene cassette 335
violacein biosynthetic pathway 337
glycosylation 316
Golden Gate assembly method 334
governance 395
green biotechnology 386

h
Halothiobacillus neapolitanus 286
helper functions, in synthetic biology 83
building blocks and structures with
DNA 87
energy 91
gene ontology 86
information 96
space management 92
time 95
homologous recombination process
34, 39

i
ICE 313
immunostimulatory therapies 352
immunosuppressive therapies 352
information technology 387
insect cell extract (ICE) 313
insertion sequences (ISs) 57
International Open Facility Advancing
Biotechnology (BioFab) 393
introns 132
isothermal assembly 265

l
Leishmania tarentolae extract 314
lipid‐based organelles 292

localizing metabolic enzymes 267

l‐threonine 242, 245

m

master function, in synthetic biology 85

Maxwell’s demon 96

MCS see multi‐cloning site (MCS)

membrane proteins 318–319

metabolic engineering 267–268

mevalonate 246–247

minimal genome 51

minimum genome factory 01

(MGF‐01) 67

mitochondrion 293

modified natural promoters,” 116–117

Moore’s Law 5–6

mRNA degradation 203

multi‐cloning site (MCS) 332

multiplex automated genome

engineering (MAGE) 27

n

nanotechnology 387

naturally evolved minimal genomes 55

natural RNA 261–262

natural yeast promoters

regulated promoters,” 113–115

ncRNA see non‐coding RNA

(ncRNA)

non‐canonical amino acids 316

non‐coding RNA (ncRNA) 193, 197

non‐homologous end joining

(NHEJ) 35, 38–39

nuclear pre‐mRNA splicing

catalytic mechanism 132

exons 132, 134

introns 132

spliceosome 132

nucleosomes 112

o

oligomerized pool engineering

(OPEN) 37

oligonucleotides (oligos) 3, 7

array‐synthesized 8

oligonucleotide‐triggered RNAi

switches 173