Index 409transcript stability control (TSC) 189
anticipating issues 201
computational design 201
confounding factor 201
genetic control mechanisms 189
model‐driven process for metabolic
pathway 198
non‐coding RNA 197
polyadenylation 195
potential mechanisms 205
probing experiment 204
RBS sequestration 203
RNA degradation process in
E.coli 190
secondary structure 196
structural and non‐coding RNA 193
translation effects 192
tuning knob 190, 195
uniformity of 5’ and 3’ ends 202
transposase fusions 42
tRNAs splicing 137
TSC see transcript stability control (TSC)
tunable trackable multiplex
recombineering (T^2 RMR) 19
u
untranslated region (UTR) 181–182, 185
upstream activation sequences
(UASs) 111
upstream repression sequences
(URSs) 113
U.S. surveys on public perception
awareness 377
vs. Europe 378
imaging 377
risk and benefits 377
risk‐benefit tradeoff 378
technology oversight 378
vs. UK public dialogue 378w
wheat germ extract (WGE) 313y
yeast extract 313
yeast promoters
core promoter 110
natural,” 113–116
nucleosomes 112
pre‐initiation complex 111
transcription activators 112
transcription factors 112
transcription initiation 112–113
upstream element 111z
zinc‐finger domains 248–249
zinc finger nucleases (ZFN) 36
zinc finger proteins 33
CoDA approach 37
delivery into cells, viral and non‐viral
methods 41
genome modification 38
modular assembly 37
OPEN selection method 37
recombinases 42
Sangamo Therapeutics, Inc. 38
Surveyor nuclease 41
transposase fusions 42
use of CRISPR/Cas9 system 38
whole exome and next generation
sequencing methods 41