Article
Extended Data Fig. 4 | Diagram of the SARS-CoV-2 pseudovirus luciferase
assay. a, Co-transfection of pNL4-3ΔEnv-nanoluc and pSARS-CoV-2 spike
vectors into 293T cells (ATCC) leads to production of SARS-CoV-2
spike-pseudotyped HIV-1 particles (SARS-CoV-2 pseudovirus) carrying the
nanoluc gene. b, SARS-CoV-2 pseudovirus is incubated for 1 h at 37 °C with
plasma or monoclonal antibody dilutions. The virus–antibody mixture is used
to infect ACE2-expressing 293T cells, which will express nanoluc luciferase
upon infection. c, Relative luminescence unit (RLU) reads from lysates of
ACE2-expressing 293T cells infected with increasing amounts of SARS-CoV-2
pseudovirus. Data are mean ± s.d. of triplicates. One representative
experiment is shown.