Article
Extended Data Fig. 1 | Bioengineering intestinal stem cell epithelia with a
tubular, in-vivo-like architecture. a, Photograph of the fully assembled
microchip system. b, Schematic cross-sectional view of laser ablation using a
nanosecond-pulsed laser. c, Hybrid collagen I/Matrigel scaffold in the central
chamber before and after microchannel ablation. Scale bars, 200 μm.
d, Fluorescence confocal images of a representative three-day old epithelial
tube. Cells are labelled with DAPI (blue, nuclei) and E-cadherin (green). Images
correspond to the maximal intensity projection of a z-stack of 100 μm. Scale
bars, 50 μm. Data are representative of at least two independent experiments.