Extended Data Fig. 2 | Establishment of shape-controlled organoid culture
from a variety of epithelial stem and progenitor cells. a, Development of bile
duct tube composed of mouse cholangiocytes. b, Fluorescence confocal
images of representative 3-day-old bile duct tubes, showing an entire tissue
(top) stained for EpCAM (orange) and a higher magnification view (bottom)
stained for actin filaments (green) and tight junction protein ZO-1 (red). Nuclei
stained with DAPI (blue). Scale bars, 50 μm. c, Development of tubular
mini-guts composed of human ISCs. d, Fluorescent confocal image showing
formation of tightly packed single-layered epithelium in 15-day-old human
mini-gut. e, Fluorescent confocal image showing proliferating Ki67+ cells
predominantly localized to the crypts in 10-day-old human mini-gut. Nuclei
and actin filaments stained with DAPI (blue) and Phalloidin (green),
respectively. Images correspond to the maximal intensity projection of a
z-stack of 80 μm. f, Formation of a human mini-airway epithelial tube and
establishment of air–liquid interface (ALI) culture from day 5 onwards. Scale
bars, 100 μm. g, Fluorescence confocal image of a representative 7-day-old
human mini-airway epithelial tube, showing an entire tissue (top) and a higher
magnification view (bottom) stained for nuclei (DAPI, blue), E-cadherin (green)
and Ki67+ proliferating cells (pink). Scale bars, 50 μm. All data are representative
of at least two independent experiments.