Article
Extended Data Fig. 4 | Plasma membrane prof iling by tandem mass tag
(TMT)-based MS3 mass spectrometry. The impact of the Dantu
polymorphism on the levels of proteins expressed on RBC membranes was
quantified using mass spectrometry. a, Hierarchical cluster analysis of all
membrane proteins, quantified and annotated as described in the Methods.
Fold change was calculated for each donor by: (signal-to-noise (donor)/average
signal-to-noise (non-Dantu)). b, Proteomic quantification of the markers shown
in Fig. 2a (three biologically independent samples per genotype group).
All markers except for GYPB were quantified by proteomics. Statistical
comparisons of quantitative protein expression across Dantu genotype
groups were performed using a two-tailed t-test with Benjamini–Hochberg
multiple hypothesis correction: *P < 0.05, **P < 0.01. All P values are listed in
Supplementary Table 4.