Nature - USA (2020-09-24)

Article

sebacatehydroxydecanoate

dodecanedioate

salicylatehydroxyoctanoate

azelate

hydroxyoctanoate

undecanedionathyladipatee

capratestearate

nonadecanoathydroxyhexanoatee

taurochenodeoxycholate

glycochenodeoxycholate

glycodeotaurolithocholatexycholate

glycolithocholate

riboth

eicosanedioate

fructose

oxypurinol

hydroxyyristate

linoleathydroexyhexadecanoate

hexadecanedioate

tetradecanedioateoleate

h

h

hyl PGA2h

hyl PGF2a

levulinate

for

arachidonateo

taurodeoxycholate

glycocholatpentadecanoate e

yristate

dodecanoate

arachidate

heptanoateheptadecanoate

hylstearate

eicosenoatenervonic acid

eicosapentaenoate (C20:5)

adrenate (C22:4)

docosahedocosapentaenoate (C22:5xaenoate (C22:6))

hydroxyphenylacetate

hydro

yristoleate

taurocholate

pentadecanolphenyllactate

eicosadienoate

02

sgNC AGPS-s

g

FA

R1-sg

OVCAR-8 free fatty acid lipidomics

b

-2 0246

0

1

2

3

4

C20:2n 6

C22:4n6

C22:6n6

C22:5n6

-1 0123456

0

1

2

3

4

C20:2nC22:4n (^66) C22:6n 6
-1 0 123456
0
1
2
3
4
5
C20:2n 6
C22:4n6
C22:6n6
C22:5n6
-2 -1 0 123456
0
1
2
3
4
Log 2 (PEX1 0 -sg / sgNC)
-Log
10
adj.
p C20:2n6
C22:6nC22:4n6 6
C22:5n6
Log 2 (PEX3-sg / sgNC)
Log 2 (AGPS-sg / sgNC) Log 2 (FAR1-sg / sgNC)
786-O free fatty acid lipidomics
-Log
10
p adj.
-Log
10
p adj.
Log2 (knockout / sgNC)
a
c
d
e
FITC / PE-TR (BODIPY-C11 510/590nm ratio)
sgNC-DMSO
sgNC-ML210
FAR1-sg2-ML210
AGPS-sg2-ML210
AGPAT3-sg1-ML210
-0.5 0 0.5 1 1.5 2
Relative frequenc
y
OVCAR-8:
Population88.2 Single Cells99.1
SSC-H
Hoechst 33342-98.7A
FSC-A SSC-A Hoechst 3334 2
SSC-A SSC-H
FITC
PE-T
R
(ox-BODIPY-C11 510nm)
(re-BODIPY-C11 590nm)
Event
s
FITC / PE-TR
(510/590nm ratio)
-5 05
0
1
2
3
4
5
Log 2 (FAR1-sg / sgNC)
C34:3 ePE
C36:4 ePE
C34:4 ePC
C42:11 ePE
C40:7 ePE
-5 -4 -3 -2 -1 0123
0
2
4
6
Log 2 (FAR1-sg / sgNC)
C38:5 ePE C34:5 ePC
C42:11 ePE
-Log
10
p adj.
ePE PUFA-TAGs
ePC diacyl-PL
OVCAR-8 lipidomics 786-O lipidomics
Extended Data Fig. 4 | See next page for caption.