Extended Data Fig. 4 | AGPS/FAR1 depletion blocks ether phospholipid
synthesis and lipid peroxidation. a, Volcano plots showing the lipidomic
analysis of OVCAR-8 and 786-O cells expressing sgNC or FA R 1-targeting
sgRNAs. n = 3 biologically independent samples. Two tailed Student’s t-test.
Multiple-testing adjustment was performed using the Benjamini–Hochberg
method. b, Heat map showing the relative abundances of free fatty acids in
wild-type and AGPS, FAR1 or PEX10-depleted OVCAR-8 cells. n = 3 biologically
independent samples. Highlighted in red are polyunsaturated fatty acids that
are enriched in response to AGPS or FA R 1 knockout. c, Volcano plots showing
free fatty acid lipidomic analysis in wild-type and AGPS, FAR1, PEX3 or PEX10-
depleted 786-O cells. n = 3 biologically independent samples. Highlighted in
red are free polyunsaturated fatty acids upregulated in the knockout cells. Two
tailed Student’s t-test. Multiple-testing adjustment was performed using the
Benjamini–Hochberg method. d, Representative gating strategy used in the
f low cytometry analysis of BODIPY-C11 oxidation levels. e, Histogram showing
the lipid peroxidation levels reported by the ratio between oxidized and
reduced BODIPY-C11 levels in the indicated OVCAR-8 cells treated with DMSO
or ML210 for 2 h. Plot of experiment performed once.